Anticorps Polyclonal de lapin anti-CEP68

• Phare
• Validé par KD/KO

CEP68 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications(8)

N° de cat : 15147-1-AP

Synonymes

Centrosomal protein of 68 kDa

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Galerie de données de validation

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  WB analysis using 15147-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15147-1-AP (CEP68 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis using 15147-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15147-1-AP (CEP68 antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15147-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15147-1-AP (CEP68 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules SH-SY5Y, cellules HeLa, cellules Jurkat

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 6 publications below
• IF: See 6 publications below

Informations sur le produit

15147-1-AP cible CEP68 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CEP68 Protéine recombinante Ag7447
• Nom complet: centrosomal protein 68kDa
• Masse moléculaire calculée: 81 kDa
• Poids moléculaire observé: 90 kDa, 67 kDa
• Numéro d’acquisition GenBank: BC002982
• Symbole du gène: CEP68
• Identification du gène (NCBI): 23177
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CEP68, or centrosomal protein 68, is a protein-coding gene in humans. It is involved in the maintenance of centrosome cohesion, likely as part of a linker structure that prevents centrosome splitting. CEP68 is also required for the localization of CDK5RAP2 to the centrosome during interphase and contributes to the formation of CROCC/rootletin filaments. This protein is encoded by the CEP68 gene and is located on the fibers emanating from the proximal ends of centrioles. It has been associated with diseases such as Retinitis Pigmentosa 28 and is implicated in angioedema. The gene is mapped to chromosome 2 and is expressed ubiquitously in various tissues, including the brain, ovary, and others. The protein plays a crucial role in cellular functions related to the centrosome, which is a key organelle in cell division and organization. This antibody recognized two bands of ∼90 and 67 kDa, respectively, in good agreement with the predicted sizes of the two Cep68 isoforms (81 and 67 kDa for Cep68L and -S, respectively).

Publications

Species	Application	Title
human	WB,IF	J Cell Sci; Cep44 functions in centrosome cohesion by stabilizing rootletin; Authors - Delowar Hossain ; KD Validated
human	WB	J Virol; The Cytoskeletal Adaptor Obscurin-Like 1 Interacts with the Human Papillomavirus 16 (HPV16) Capsid Protein L2 and Is Required for HPV16 Endocytosis.; Authors - Elena Wüstenhagen ; KD Validated
human	IF	Sci Rep; Prolonged overexpression of PLK4 leads to formation of centriole rosette clusters that are connected via canonical centrosome linker proteins; Authors - Selahattin Can Ozcan
human	WB,IF	J Cell Sci; Cep44 functions in centrosome cohesion by stabilizing rootletin.; Authors - Delowar Hossain ; KD Validated
human	WB	RNA; Human Nuclear RNAi-Defective 2 (NRDE2) is an essential RNA splicing factor.; Authors - Alan L Jiao
human	WB,IF	iScience; Pathogenic LRRK2 regulates centrosome cohesion via Rab10/RILPL1-mediated CDK5RAP2 displacement.; Authors - Elena Fdez

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