Anticorps Monoclonal anti-Cofilin

• Phare
• Validé par KD/KO

Cofilin Monoclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, Lapin, porc, rat, souris

Applications

WB, IHC, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(25)

CloneNo.

1G6A2

Avis (2)

N° de cat : 66057-1-Ig

Synonymes

CFL1, Cofilin1, Cofilin, non-muscle isoform, Cofilin 1, 1G6A2

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Galerie de données de validation

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  WB analysis using 66057-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66057-1-Ig (Cofilin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66057-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66057-1-Ig (Cofilin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 66057-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66057-1-Ig (Cofilin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human testis using 66057-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis using 66057-1-Ig(CFL1 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human testis using 66057-1-Ig

  Immunohistochemical analysis of paraffin-embedded human testis using 66057-1-Ig(CFL1 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human lung cancer using 66057-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66057-1-Ig (Cofilin antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung cancer using 66057-1-Ig

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 66057-1-Ig (Cofilin antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 66057-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66057-1-Ig (Cofilin antibody) at dilution of 1:500 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 66057-1-Ig

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 66057-1-Ig (Cofilin antibody) at dilution of 1:500 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IF Staining of HeLa using 66057-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Cofilin antibody (66057-1-Ig, Clone: 1G6A2 ) at dilution of 1:800 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) (SA00013-1), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules LNCaP, cellules A431, cellules HEK-293, cellules HeLa, cellules Jurkat, cellules K-562, cellules Neuro-2a, cellules PC-12, cellules SH-SY5Y, tissu cérébral de lapin, tissu cérébral de porc, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IHC: tissu testiculaire humain, tissu de cancer du poumon humain, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 24 publications below
• IF: See 6 publications below
• IP: See 2 publications below

Informations sur le produit

66057-1-Ig cible Cofilin dans les applications de WB, IHC, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, Lapin, porc, rat, souris

• Réactivité: Humain, Lapin, porc, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Cofilin Protéine recombinante Ag18044
• Nom complet: cofilin 1 (non-muscle)
• Masse moléculaire calculée: 18 kDa
• Poids moléculaire observé: 18 kDa
• Numéro d’acquisition GenBank: BC012318
• Symbole du gène: Cofilin
• Identification du gène (NCBI): 1072
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cofilin is a ubiquitous actin-binding protein required for the reorganization of actin filaments. It is a member of ADF (actin-depolymerizing factor)/cofilin family that is a key regulator of actin dynamics and essential for cellular motility, cytokinesis, and endocytosis. Cofilin activity is tightly regulated by phosphorylation and dephosphorylation. Phosphorylation at Ser3 can inhibit its activity, also causing translocation from the nucleus to the cytoplasm.

Publications

Species	Application	Title
	WB,IF,IP	Nat Commun; N-terminal α-amino SUMOylation of cofilin-1 is critical for its regulation of actin depolymerization; Authors - Weiji Weng
mouse	WB	Mol Psychiatry; Phosphoproteomic of the acetylcholine pathway enables discovery of the PKC-β-PIX-Rac1-PAK cascade as a stimulatory signal for aversive learning.; Authors - Yukie Yamahashi
human	WB	Nat Commun; Nuclear-capture of endosomes depletes nuclear G-actin to promote SRF/MRTF activation and cancer cell invasion.; Authors - Sergi Marco
mouse	WB	Nat Commun; Dendritic autophagy degrades postsynaptic proteins and is required for long-term synaptic depression in mice.; Authors - Emmanouela Kallergi
mouse	WB	Elife; Giant ankyrin-B mediates transduction of axon guidance and collateral branch pruning factor sema 3A; Authors - Blake A Creighton
human	WB	Int J Nanomedicine; Protein Nanoparticle-Related Osmotic Pressure Modifies Nonselective Permeability of the Blood-Brain Barrier by Increasing Membrane Fluidity.; Authors - Chen Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Udesh (Verified Customer) (08-19-2024)

Worked well at 1:3000

• Applications: Western Blot
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: HEK293T

Cofilin Antibody Western Blot validation (1:3000 dilution) in HEK293T (Cat no:66057-1-Ig)

FH

Marina (Verified Customer) (03-12-2024)

Samples are two tumour (glioblastoma) cell lines (TC1, TC2). Gradient gel 4-15 %. Dilution 1:5000, primary antibody incubation 4ºC overnight, secondary antibody incubation 1 h room temperature. The bands appear at the expected size of the target protein.

• Applications: Western Blot
• Primary Antibody Dilution: 1:5000
• Cell Tissue Type: Human tumour cell lines

Cofilin Antibody Western Blot validation (1:5000 dilution) in Human tumour cell lines (Cat no:66057-1-Ig)

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