Anticorps Polyclonal de lapin anti-Cingulin

• Phare
• Validé par KD/KO

Cingulin Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(12)

N° de cat : 21369-1-AP

Synonymes

KIAA1319, CGN

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Galerie de données de validation

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  WB analysis using 21369-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21369-1-AP (Cingulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 21369-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 21369-1-AP (Cingulin antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 21369-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21369-1-AP (Cingulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse small intestine using 21369-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 21369-1-AP (Cingulin antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse kidney using 21369-1-AP

  IP result of anti-Cingulin (IP:21369-1-AP, 4ug; Detection:21369-1-AP 1:800) with mouse kidney tissue lysate 4800ug.

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  IHC staining of human ovary cancer using 21369-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 21369-1-AP (Cingulin antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293T, cellules MCF-7, tissu d'intestin grêle de souris, tissu rénal de souris
• Résultats positifs en IP: tissu rénal de souris
• Résultats positifs en IHC: human ovary cancer tissue, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IHC: See 1 publications below
• IF: See 7 publications below
• IP: See 1 publications below

Informations sur le produit

21369-1-AP cible Cingulin dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, canin, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Cingulin Protéine recombinante Ag15843
• Nom complet: cingulin
• Masse moléculaire calculée: 1197 aa, 136 kDa
• Poids moléculaire observé: 136-150 kDa
• Numéro d’acquisition GenBank: BC146657
• Symbole du gène: Cingulin
• Identification du gène (NCBI): 57530
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cingulin is a component of the cytoplasmic plaque of tight junction, and is localized on the cytoplasmic face of tight junctions of polarized epithelia and some endothelia. Cingulin is a homodimer, each subunit containing a globular "head" domain, a coiled-coil "rod" domain and a small globular "tail" region. Cingulin can interact interact with ZO-1, ZO-2, ZO-3, actin, and myosin. It probably plays a role in the formation and regulation of the tight junction paracellular permeability barrier.

Publications

Species	Application	Title
human	WB	Nat Commun; The tight junction protein TJP1 regulates the feeding-modulated hepatic circadian clock.; Authors - Yi Liu
canine	WB,IF	Nat Commun; Cingulin and actin mediate midbody-dependent apical lumen formation during polarization of epithelial cells.; Authors - Anthony J Mangan ; KO Validated
mouse	WB,IF	Mucosal Immunol; Epithelial NELF guards intestinal barrier function to ameliorate colitis by maintaining junctional integrity.; Authors - Jiayao Ou
human	WB,IHC	Cancers (Basel); miR-125b Promotes Colorectal Cancer Migration and Invasion by Dual-Targeting CFTR and CGN.; Authors - Xiaohui Zhang
rat	WB	Sci Rep; Treatment with Ligilactobacillus murinus lowers blood pressure and intestinal permeability in spontaneously hypertensive rats; Authors - Masashi Mukohda
mouse	WB, IP	Cell Signal; Motif affinity and mass spectrometry proteomic approach for the discovery of cellular AMPK targets: Identification of mitochondrial fission factor as a new AMPK substrate.; Authors - Serge Ducommun

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