Anticorps Polyclonal de lapin anti-CHAF1B

• Phare
• Validé par KD/KO

CHAF1B Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ChIP, ELISA

Conjugaison

Non conjugué

Publications

2

N° de cat : 27633-1-AP

Synonymes

CAF 1, CAF 1 subunit B, CAF I 60 kDa subunit, CAF I p60, CAF IP60, CAF1, CAF1A, CAF1P60, CHAF1B, M phase phosphoprotein 7, MPHOSPH7, MPP7

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Galerie de données de validation

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  WB analysis using 27633-1-AP

  Various cells were subjected to SDS PAGE followed by western blot with 27633-1-AP (CHAF1B antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 27633-1-AP

  HepG2 cells were subjected to SDS PAGE followed by western blot with 27633-1-AP (CHAF1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 27633-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 27633-1-AP (CHAF1B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules Caco-2, cellules HeLa, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• ChIP: See 1 publications below

Informations sur le produit

27633-1-AP cible CHAF1B dans les applications de WB, ChIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CHAF1B Protéine recombinante Ag26504
• Nom complet: chromatin assembly factor 1, subunit B (p60)
• Masse moléculaire calculée: 559 aa, 61 kDa
• Poids moléculaire observé: 65-70 kDa
• Numéro d’acquisition GenBank: BC021218
• Symbole du gène: CHAF1B
• Identification du gène (NCBI): 8208
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CHAF1B, also named as CAF1A, CAF1P60, MPHOSPH7, MPP7, is a 559 amino acid protein, which belongs to the WD repeat HIR1 family. CHAF1B as a subunit of the CAF-1 complex that contains RBBP4, CHAF1B and CHAF1A. CHAF1B is thought to mediate chromatin assembly in DNA replication and DNA repair and assembles histone octamers onto replicating DNA in vitro. The calcualted molecular weight of CHAF1B is 61 kDa, but molecular weight of CHAF1B is detected about 65-70 kDa.

Publications

Species	Application	Title
human	WB	Biomed Pharmacother; Affinity-based protein profiling to reveal targets of puerarin involved in its protective effect on cardiomyocytes.; Authors - Shuai Huang ; KD Validated
human	ChIP	Cancer Res Commun; Targeting CHAF1B Enhances IFN Activity against Myeloproliferative Neoplasm Cells; Authors - Diana Saleiro