Anticorps Polyclonal de lapin anti-CHCHD2

• Phare
• Validé par KD/KO

CHCHD2 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

30

N° de cat : 19424-1-AP

Synonymes

C7orf17, CHCHD2, MNRR1, NS2TP

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Galerie de données de validation

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  WB analysis using 19424-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19424-1-AP (CHCHD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 19424-1-AP

  WB result of CHCHD2 antibody (19424-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHCHD2 transfected HEK-293 cells.

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  WB analysis using 19424-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19424-1-AP (CHCHD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human adrenal gland using 19424-1-AP

  human adrenal gland tissue were subjected to SDS PAGE followed by western blot with 19424-1-AP (CHCHD2 antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

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  IP experiment of HepG2 using 19424-1-AP

  IP result of anti-CHCHD2 (IP:19424-1-AP, 4ug; Detection:19424-1-AP 1:1000) with HepG2 cells lysate 2520 ug.

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  IHC staining of human lung cancer using 19424-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19424-1-AP (CHCHD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human lung cancer using 19424-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung cancer tissue slide using 19424-1-AP (CHCHD2 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 19424-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19424-1-AP (CHCHD2 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of mouse brain using 19424-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 19424-1-AP (CHCHD2 Antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HepG2 using 19424-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CHCHD2 antibody (19424-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  IF Staining of HepG2 using 19424-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CHCHD2 antibody (19424-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 19424-1-AP

  Immunofluorescent analysis of HepG2 cells, using CHCHD2 antibody 19424-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: cellules HT-1080, cellules HEK-293, cellules HepG2, tissu de glande surrénale humain
• Résultats positifs en IP: cellules HepG2,
• Résultats positifs en IHC: tissu de cancer du poumon humain, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 27 publications below
• IHC: See 2 publications below
• IF: See 6 publications below
• CoIP: See 2 publications below

Informations sur le produit

19424-1-AP cible CHCHD2 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Drosophile, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CHCHD2 Protéine recombinante Ag13752
• Nom complet: coiled-coil-helix-coiled-coil-helix domain containing 2
• Masse moléculaire calculée: 151 aa, 16 kDa
• Poids moléculaire observé: 16-18 kDa
• Numéro d’acquisition GenBank: BC003079
• Symbole du gène: CHCHD2
• Identification du gène (NCBI): 51142
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CHCHD2 is a widely expressed 16.7-kDa mitochondrion-localized protein. CHCHD2 contains a C-terminal CHCH (coiled-coil helix coiled-coil helix) domain. Mutations in CHCHD2 gene have been reported in autosomal dominant Parkinson's disease (ADPD). CHCHD2 is a bi-organellar mediator of oxidative phosphorylation, playing crucial roles in regulating electron flow in the mitochondrial electron transport chain and acting as a nuclear transcription factor for a cytochrome c oxidase subunit (COX4I2) and itself in response to hypoxic stress. CHCHD2 also regulates cell migration and differentiation, mitochondrial cristae structure, and apoptosis (PMID: 33967741).

Publications

Species	Application	Title
human	WB, IF	Lancet Neurol; CHCHD2 mutations in autosomal dominant late-onset Parkinson's disease: a genome-wide linkage and sequencing study.; Authors - Manabu Funayama
Drosophila	WB	Nat Commun; Loss of Parkinson's disease-associated protein CHCHD2 affects mitochondrial crista structure and destabilizes cytochrome c.; Authors - Hongrui Meng
human	WB	Nat Commun; TDP-43 and PINK1 mediate CHCHD10S59L mutation-induced defects in Drosophila and in vitro.; Authors - Minwoo Baek
human	WB	Proc Natl Acad Sci U S A; Mitochondrial Nuclear Retrograde Regulator 1 (MNRR1) rescues the cellular phenotype of MELAS by inducing homeostatic mechanisms.; Authors - Siddhesh Aras
human	IHC,IF	Neurology; Mitochondrial targeting sequence variants of the CHCHD2 gene are a risk for Lewy body disorders.; Authors - Kotaro Ogaki
human	WB	Nucleic Acids Res; Oxygen-dependent expression of cytochrome c oxidase subunit 4-2 gene expression is mediated by transcription factors RBPJ, CXXC5 and CHCHD2.; Authors - Aras Siddhesh S

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