Anticorps Recombinant de lapin anti-CHCHD2

• Phare
• Validé par KD/KO

CHCHD2 Recombinant Antibody for WB, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat

Applications

WB, IF/ICC, ELISA

Conjugaison

Non conjugué

CloneNo.

4A12

Avis (1)

N° de cat : 82702-1-RR

Synonymes

HCV NS2 trans-regulated protein, Coiled-coil-helix-coiled-coil-helix domain-containing protein 2, C7orf17, Aging-associated gene 10 protein, AAG10

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Galerie de données de validation

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  WB analysis using 82702-1-RR

  Various lysates were subjected to SDS PAGE followed by western blot with 82702-1-RR (CHCHD2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 82702-1-RR

  WB result of CHCHD2 antibody (82702-1-RR; 1:2000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CHCHD2 transfected HEK-293 cells.

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  IF Staining of HepG2 using 82702-1-RR

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CHCHD2 antibody (82702-1-RR, Clone: 4A12 ) at dilution of 1:500 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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  Affinity and Kinetic Characterization of 82702-1-RR

  Biolayer interferometry (BLl) kinetic assays of 82702-1-RR against Human CHCHD2 were performed. The affinity constant is 2.12 nM.

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HSC-T6, cellules Jurkat, cellules K-562, cellules LNCaP
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF)/ICC: IF/ICC : 1:250-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

82702-1-RR cible CHCHD2 dans les applications de WB, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat

• Réactivité: Humain, rat
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CHCHD2 Protéine recombinante Ag13752
• Nom complet: coiled-coil-helix-coiled-coil-helix domain containing 2
• Masse moléculaire calculée: 151 aa, 16 kDa
• Poids moléculaire observé: 16 kDa
• Numéro d’acquisition GenBank: BC003079
• Symbole du gène: CHCHD2
• Identification du gène (NCBI): 51142
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CHCHD2 is a widely expressed 16.7-kDa mitochondrion-localized protein. CHCHD2 contains a C-terminal CHCH (coiled-coil helix coiled-coil helix) domain. Mutations in CHCHD2 gene have been reported in autosomal dominant Parkinson's disease (ADPD). CHCHD2 is a bi-organellar mediator of oxidative phosphorylation, playing crucial roles in regulating electron flow in the mitochondrial electron transport chain and acting as a nuclear transcription factor for a cytochrome c oxidase subunit (COX4I2) and itself in response to hypoxic stress. CHCHD2 also regulates cell migration and differentiation, mitochondrial cristae structure, and apoptosis (PMID: 33967741).

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Vignesh (Verified Customer) (08-28-2025)

It's an excellent product with reduced or no nonspecific bands.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: HTB126

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