Anticorps Polyclonal de lapin anti-CHCHD4

• Phare
• Validé par KD/KO

CHCHD4 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

25

Avis (3)

N° de cat : 21090-1-AP

Synonymes

CHCHD4, MIA40

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Galerie de données de validation

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  WB analysis of Daudi using 21090-1-AP

  Daudi cells were subjected to SDS PAGE followed by western blot with 21090-1-AP (CHCHD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of Raji using 21090-1-AP

  Raji cells were subjected to SDS PAGE followed by western blot with 21090-1-AP (CHCHD4 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat brain using 21090-1-AP

  rat brain tissue were subjected to SDS PAGE followed by western blot with 21090-1-AP (CHCHD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of Ramos using 21090-1-AP

  Ramos cells were subjected to SDS PAGE followed by western blot with 21090-1-AP (CHCHD4 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 21090-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 21090-1-AP (CHCHD4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 21090-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 21090-1-AP (CHCHD4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 21090-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 21090-1-AP (CHCHD4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of L02 using 21090-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 21090-1-AP (CHCHD4 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 21090-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 21090-1-AP (CHCHD4 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 21090-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 21090-1-AP (CHCHD4 antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse brain using 21090-1-AP

  IP result of anti-CHCHD4 (IP:21090-1-AP, 4ug; Detection:21090-1-AP 1:1000) with mouse brain tissue lysate 3200 ug.

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  IHC staining of mouse testis using 21090-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21090-1-AP (CHCHD4 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 21090-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 21090-1-AP (CHCHD4 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 21090-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21090-1-AP (CHCHD4 antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human kidney using 21090-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 21090-1-AP (CHCHD4 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of A431 using 21090-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CHCHD4 antibody (21090-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules Daudi, cellules L02, cellules Raji, cellules Ramos, tissu cardiaque humain, tissu cérébral de rat, tissu cérébral de souris, tissu pulmonaire de souris, tissu rénal de souris, tissu splénique de souris
• Résultats positifs en IP: tissu cérébral de souris,
• Résultats positifs en IHC: tissu testiculaire de souris, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 23 publications below
• IHC: See 1 publications below
• IF: See 3 publications below
• IP: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

21090-1-AP cible CHCHD4 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CHCHD4 Protéine recombinante Ag15339
• Nom complet: coiled-coil-helix-coiled-coil-helix domain containing 4
• Masse moléculaire calculée: 142 aa, 16 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC033775
• Symbole du gène: CHCHD4
• Identification du gène (NCBI): 131474
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CHCHD4, a component of human mitochondria, belongs to a protein family. It functions as chaperone and catalyzes the formation of disulfide bonds in substrate proteins, such as COX17. It is required for the import and folding of small cysteine-containing proteins (small Tim) in the mitochondrial intermembrane space (IMS).

Publications

Species	Application	Title
human	WB	Cell Stem Cell; Disrupting Mitochondrial Copper Distribution Inhibits Leukemic Stem Cell Self-Renewal.; Authors - Rashim Pal Singh
rat	WB,IF	Exp Mol Med; SIRT5-related desuccinylation modification of AIFM1 protects against compression-induced intervertebral disc degeneration by regulating mitochondrial homeostasis; Authors - Jianxin Mao
human	WB,IF	Aging Cell; Dependence of PINK1 accumulation on mitochondrial redox system.; Authors - Feng Gao ; KD Validated
human	WB	Redox Biol; Flow-induced endothelial mitochondrial remodeling mitigates mitochondrial reactive oxygen species production and promotes mitochondrial DNA integrity in a p53-dependent manner.; Authors - Junchul Shin
human	WB	EMBO Mol Med; A novel CHCHD10 mutation implicates a Mia40-dependent mitochondrial import deficit in ALS.; Authors - Carina Lehmer
human	WB	Oncogene; CHTM1, a novel metabolic marker deregulated in human malignancies.; Authors - Mansi Babbar

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Veronica (Verified Customer) (10-17-2023)

First time I find an anti-CHCHD4 antibody that works fine. We are testing it in IF as well, but till now I am already really happy with the result in WB. No aspecific signal, clear band. Reccomended! Conditions: Gradient gel 8-15% Ab’: 1:1000 in 5% milk O/N

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293T; HeLa; isolated mitochondria from HEK293T

CHCHD4 Antibody Western Blot validation (1:1000 dilution) in HEK293T; HeLa; isolated mitochondria from HEK293T (Cat no:21090-1-AP)

FH

Süleyman (Verified Customer) (01-23-2022)

CHCHD4 antibody (Green) is used 1:100 dilution (2 hour at RT) and 1:700 of Alexa Fluor 488 secondary antibody (1 hour RT). 4% Paraformaldehyde fixation for 10 min at RT. Permeabilization with 0.3% Triton X for 10-15 minutes at RT. 5% FBS in PBS used for blocking for 1 hour at RT. The image is colocalized with TOMM20 (Red). Blue is DAPI. As you can see there is colocalization with TOMM20 as CHCHD4 supposed to be in IMS but I also see unspecific bindings in cytosolic proteins. Therefore my rating is 3 as it might be binding unspecifically.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:100
• Cell Tissue Type: HeLa FlipIn

CHCHD4 Antibody Immunofluorescence validation (1:100 dilution) in HeLa FlipIn (Cat no:21090-1-AP)

FH

Lana (Verified Customer) (08-13-2019)

SDS-PAGE: 10 ug/ul RIPA lysate of the mitochondrial fraction of HEK293t. 4-12% Bis-tris gradient gel.Transfer: Immobilon-FL transfer membranes (Millipore) O/N at 30V, 4CBlocking: SEA Block Blocking Buffer 1hPrimary Ab: O/N incubation at 4CSecondary Ab: IRDye 800CW Goat anti-Rabbit

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HEK293t

CHCHD4 Antibody Western Blot, validation (1:1000 dilution) in HEK293t (Cat no:21090-1-AP)