Anticorps Polyclonal de lapin anti-CHD9

• Phare
• Validé par KD/KO

CHD9 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ChIP, ELISA

Conjugaison

Non conjugué

Publications(4)

N° de cat : 13402-1-AP

Synonymes

AD013, AD-013, ATP-dependent helicase CHD9, CHD-9, Chromatin-related mesenchymal modulator

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Galerie de données de validation

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  WB analysis using 13402-1-AP

  SGC-7901 cells were subjected to SDS PAGE followed by western blot with 13402-1-AP (CHD9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 13402-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 13402-1-AP (CHD9 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 13402-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 13402-1-AP (CHD9 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 13402-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 13402-1-AP (CHD9 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of Hela using 13402-1-AP

  Immunofluorescent analysis of (4% PFA) fixed Hela cells using CHD9 antibody (13402-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules SGC-7901, cellules K-562
• Résultats positifs en IHC: tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 2 publications below
• IHC: See 3 publications below
• ChIP: See 1 publications below

Informations sur le produit

13402-1-AP cible CHD9 dans les applications de WB, IHC, IF/ICC, ChIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CHD9 Protéine recombinante Ag4245
• Nom complet: chromodomain helicase DNA binding protein 9
• Masse moléculaire calculée: 2897 aa, 326 kDa
• Poids moléculaire observé: 330 kDa
• Numéro d’acquisition GenBank: BC033770
• Symbole du gène: CHD9
• Identification du gène (NCBI): 80205
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CHD (chromodomain helicase DNA-binding) proteins are chromatin remodelling enzymes which reshape the local chromatin architecture to allow access to transcriptional machinery in a tissue-specific context. CHD proteins facilitate various stages of gene expression, including binding and recruiting transcription factors, histone modifications, and transcriptional elongation, termination and processing. CHD proteins are characterized by two tandem chromodomains and a helicase C domain, but also possesses additional functional domains which classify them into three subfamilies. CHD9 is active in mesenchymal stromal stem cells and osteoprogenitor cells, and is suggested to control osteogenic cell fate (PMID: 32295522).

Publications

Species	Application	Title
human	WB,IHC	Mol Carcinog; miR-200c-3p spreads invasive capacity in human oral squamous cell carcinoma microenvironment.; Authors - Tomoyo Kawakubo-Yasukochi ; KD Validated
human	IHC	Braz J Med Biol Res; Decreased expression of chromodomain helicase DNA-binding protein 9 is a novel independent prognostic biomarker for colorectal cancer.; Authors - Li Xu
human	IHC	Int J Clin Exp Pathol; High CHD9 expression is associated with poor prognosis in clear cell renal cell carcinoma.; Authors - Bo Guan
human	WB,ChIP	mBio; A Two-Color Haploid Genetic Screen Identifies Novel Host Factors Involved in HIV-1 Latency.; Authors - Michael Röling ; KD Validated

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