Anticorps Polyclonal de lapin anti-CHDH

• Phare
• Validé par KD/KO

CHDH Polyclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

Avis (2)

N° de cat : 17356-1-AP

Synonymes

CDH, CHD, CHDH, Choline dehydrogenase

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Galerie de données de validation

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  WB analysis using 17356-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 17356-1-AP (CHDH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 17356-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17356-1-AP (CHDH antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 17356-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 17356-1-AP (CHDH antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver cancer using 17356-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17356-1-AP (CHDH antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 17356-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 17356-1-AP (CHDH antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu hépatique de rat, tissu hépatique de souris, tissu rénal de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:6000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 1 publications below
• IHC: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

17356-1-AP cible CHDH dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CHDH Protéine recombinante Ag10614
• Nom complet: choline dehydrogenase
• Masse moléculaire calculée: 594 aa, 65 kDa
• Poids moléculaire observé: 60 kDa
• Numéro d’acquisition GenBank: BC034502
• Symbole du gène: CHDH
• Identification du gène (NCBI): 55349
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CHDH(Choline dehydrogenase, mitochondrial) is also named as CDH,CHD and belongs to the GMC oxidoreductase family. CHDH is a member of the glucose-methanol-choline family of oxidoreductases, with each member containing a consensus FAD-binding domain. Many members of this oxidoreductase family are soluble proteins but mammalian CHDH is associated with the inner mitochondrial membrane(Handbook of vitamins,Robert B,2007). The full length protein(65 kDa) has a transit peptide of 29 amino acid and the mature CHDH is appropriately located in the inner mitochondria membrane(PMID:12951056). 17356-1-AP can also recognize the 45 kDa isoform X3 of CHDH.

Publications

Species	Application	Title
mouse	WB	J Biol Chem; Betaine is accumulated via transient choline dehydrogenase activation during Mouse oocyte meiotic maturation.; Authors - Taylor McClatchie ; KO Validated
human	IHC	PLoS One; Choline dehydrogenase polymorphism rs12676 is a functional variation and is associated with changes in human sperm cell function.; Authors - Johnson Amy R AR
human	IHC	Transl Oncol; Quantitative proteomic profiling of hepatocellular carcinoma at different serum alpha-fetoprotein level.; Authors - Xuyong Wei
human	IF	Sci Adv; Maintaining hypoxia environment of subchondral bone alleviates osteoarthritis progression; Authors - Hao Zhang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Wojciech (Verified Customer) (03-23-2023)

A band was detected at around 60 kDa. 30 ug of protein was loaded and probed with the antibody at 1:2000 dilution.

• Applications: Western Blot
• Primary Antibody Dilution: 1:2000
• Cell Tissue Type: T-ALL cell line (CCRF-CEM)

FH

Hua (Verified Customer) (12-04-2018)

40 ug of mouse liver lysate was loaded onto the gel. The incubation time is over night at 4oC.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse liver

CHDH Antibody Western Blot, validation (1:1000 dilution) in mouse liver (Cat no:17356-1-AP)