Anticorps Polyclonal de lapin anti-Chromogranin B

• Phare
• Validé par KD/KO

Chromogranin B Polyclonal Antibody for WB, IHC, IF-P, IF-Fro, FC (Intra), ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF-P, IF-Fro, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(11)

Avis (2)

N° de cat : 14968-1-AP

Synonymes

CgB, CHGB, SCG1, Chromogranin-B, PE-11

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Galerie de données de validation

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  WB analysis using 14968-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14968-1-AP (Chromogranin B antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of PC-12 using 14968-1-AP

  PC-12 cells were subjected to SDS PAGE followed by western blot with 14968-1-AP (Chromogranin B antibody at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 14968-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 14968-1-AP (Chromogranin B antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 14968-1-AP

  RAW 264.7 cells were subjected to SDS PAGE followed by western blot with 14968-1-AP (Chromogranin B antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human pancreas using 14968-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14968-1-AP (Chromogranin B antibody at dilution of 1:200 (under 10x lens).

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  IHC staining of human pancreas using 14968-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas tissue slide using 14968-1-AP (Chromogranin B antibody at dilution of 1:200 (under 40x lens).

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  IHC staining of mouse pancreas using 14968-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 14968-1-AP (Chromogranin B antibody) at dilution of 1:400 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse pancreas using 14968-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse pancreas tissue slide using 14968-1-AP (Chromogranin B antibody) at dilution of 1:400 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse colon using 14968-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using Chromogranin B antibody (14968-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse colon using 14968-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse colon tissue using Chromogranin B antibody (14968-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse colon using 14968-1-AP

  Immunofluorescent analysis of (4% PFA) fixed frozen OCT-embedded mouse colon tissue using 14968-1-AP (Chromogranin B antibody) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  FC experiment of PC-12 using 14968-1-AP

  1X10^6 PC-12 cells were intracellularly stained with 0.4 ug Anti-Human Chromogranin B (14968-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) at dilution 1:1000 (red), or 0.4 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules NIH/3T3, cellules RAW 264.7, tissu cérébral de souris
• Résultats positifs en IHC: tissu pancréatique humain, tissu pancréatique de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de côlon de souris,
• Résultats positifs en IF-Fro: tissu de côlon de souris,
• Résultats positifs en FC (Intra): cellules PC-12,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)-FRO: IF-FRO : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 6 publications below
• IHC: See 1 publications below
• IF: See 2 publications below

Informations sur le produit

14968-1-AP cible Chromogranin B dans les applications de WB, IHC, IF-P, IF-Fro, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Chromogranin B Protéine recombinante Ag6833
• Nom complet: chromogranin B (secretogranin 1)
• Masse moléculaire calculée: 78 kDa
• Poids moléculaire observé: 70-100 kDa
• Numéro d’acquisition GenBank: BC000375
• Symbole du gène: Chromogranin B
• Identification du gène (NCBI): 1114
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Chromogranin B is also named as Secretogranin-1, SgI, CgB, CHGB and SCG1. CHGB, a secretory granule protein, inserts itself into membrane and forms a chloride-conducting channel (PMID: 30456382). Native CHGB distributes nearly equally in soluble and membrane-bound forms, and both reconstitute highly selective anion channels in membrane (PMID: 37435575). The calculated molecular weight of Chromogranin B is at 78kDa. It has been shown that CHGB has a clear degradation band at ~50 kDa (https://www.biorxiv.org/content/10.1101/2019.12.28.890053v1.full.pdf).

Publications

Species	Application	Title
human	WB	Adv Sci (Weinh); Identification of PRDX5 as A Target for The Treatment of Castration-Resistant Prostate Cancer; Authors - Rong Wang
		J Cell Sci; PMID: 31182646; Authors - Shelby C Bearrows
human	WB	J Virol; Kaposi's Sarcoma-Associated Herpesvirus Infection Induces the Expression of Neuroendocrine Genes in Endothelial Cells.; Authors - Mohanan Valiya Veettil
human	WB	J Proteome Res; Label-Free LC-MS/MS Proteomic Analysis of Cerebrospinal Fluid Identifies Protein/Pathway Alterations and Candidate Biomarkers for Amyotrophic Lateral Sclerosis.; Authors - Mahlon Angus Collins
mouse	WB	Biosci Biotechnol Biochem; Identification of proteins that bind to the neuroprotective agent neoechinulin A.; Authors - Shinji Kamisuki ; KD Validated
mouse	IF	mBio; Citrobacter rodentium Infection Induces Persistent Molecular Changes and Interferon Gamma-Dependent Major Histocompatibility Complex Class II Expression in the Colonic Epithelium.; Authors - Caroline Mullineaux-Sanders

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Patricia (Verified Customer) (05-15-2025)

I used the antibody to stain cells within the Islets of Langerhans in pancreatic tissue from organ donors, and it worked very well in combination with AF647.

• Applications: Immunohistochemistry
• Primary Antibody Dilution: 1:700
• Cell Tissue Type: Human pancras

Chromogranin B Antibody Immunohistochemistry validation (1:700 dilution) in Human pancras (Cat no:14968-1-AP)

FH

Julia (Verified Customer) (03-14-2022)

Works very well after 1 hour incubation in PBS-T supplemented with normal donkey serum (secondary also added for an hour). Very clear staining.

• Applications: Immunofluorescence
• Primary Antibody Dilution: 1:50
• Cell Tissue Type: Mouse colonic tissue

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