Anticorps Polyclonal de lapin anti-CHI3L1/YKL40

• Phare
• Validé par KD/KO

CHI3L1/YKL40 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

14

N° de cat : 12036-1-AP

Synonymes

39 kDa synovial protein, ASRT7, Cartilage glycoprotein 39, CGP 39, CHI3L1, CHI3L1/YKL40, Chitinase 3 like protein 1, Chitinase 3-like 1, GP 39, GP39, HC gp39, hCGP 39, HCGP 3P, YKL 40, YKL40, YYL 40

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Galerie de données de validation

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  WB analysis of THP-1 using 12036-1-AP

  THP-1 cells were subjected to SDS PAGE followed by western blot with 12036-1-AP (CHI3L1/YKL40 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of THP-1 using 12036-1-AP

  WB result of CHI3L1/YKL40 antibody (12036-1-AP; 1:10000; room temperature for 1.5 hours) with wild-type and CHI3L1/YKL40 knockout THP-1 cells.

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  IHC staining of human liver using 12036-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12036-1-AP (CHI3L1 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver using 12036-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver tissue slide using 12036-1-AP (CHI3L1 Antibody) at dilution of 1:200 (under 40x lens).

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  IHC staining of human ovary tumor using 12036-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 12036-1-AP (CHI3L1 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human ovary tumor using 12036-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor using 12036-1-AP (CHI3L1 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of mouse brain using 12036-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CHI3L1/YKL40 antibody (12036-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse brain using 12036-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using CHI3L1/YKL40 antibody (12036-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules THP-1,
• Résultats positifs en IHC: tissu hépatique humain, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu cérébral de souris,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:100-1:400
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 5 publications below
• IHC: See 7 publications below
• IF: See 5 publications below

Informations sur le produit

12036-1-AP cible CHI3L1/YKL40 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CHI3L1/YKL40 Protéine recombinante Ag2710
• Nom complet: chitinase 3-like 1 (cartilage glycoprotein-39)
• Masse moléculaire calculée: 383 aa, 43 kDa
• Poids moléculaire observé: 40, 33 kDa
• Numéro d’acquisition GenBank: BC008568
• Symbole du gène: CHI3L1
• Identification du gène (NCBI): 1116
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Chitinase 3-like-1 (CHI3L1/YKL-40/CGP-39/GP-39/hCGP-39) is a protein secreted from restricted cell types including colonic epithelial cells (CECs) and macrophages. CHI3L1 is an inflammation-associated molecule, and its expression is enhanced in persons with colitis and colon cancer. CHI3L1 is a 40 kDa protein and is produced by restricted cell types, including colonic epithelial cells (CECs) and macrophages. CHI3L1 can be detected in the Golgi apparatus and the endoplasmic reticulum, but its major sites of action seems to be extracellular as a secreted protein. (PMID:21763261)

Publications

Species	Application	Title
human	WB	J Neurol Neurosurg Psychiatry; Molecules of senescent glial cells differentiate Alzheimer's disease from ageing; Authors - Linbin Dai
mouse	IHC, IF, WB	Elife; Chitinase 3-like-1 contributes to acetaminophen-induced liver injury by promoting hepatic platelet recruitment.; Authors - Zhao Shan
human	IHC	Genomics; MIN score predicts primary response to infliximab/adalimumab and vedolizumab therapy in patients with inflammatory bowel diseases.; Authors - Yuan Shi
human	IF	Glia; SSEA-4 and YKL-40 positive progenitor subtypes in the subventricular zone of developing human neocortex.; Authors - Christian B Brøchner
human	IHC	Front Oncol; BYSL Promotes Glioblastoma Cell Migration, Invasion, and Mesenchymal Transition Through the GSK-3β/β-Catenin Signaling Pathway.; Authors - Zhuang Sha
mouse	IHC	Pharmaceutics; Chitooligosaccharides Improve the Efficacy of Checkpoint Inhibitors in a Mouse Model of Lung Cancer.; Authors - Astrid Zedlitz Johansen