Anticorps Polyclonal de lapin anti-Choline Kinase Alpha

• Phare
• Validé par KD/KO

Choline Kinase Alpha Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

21

Avis (1)

N° de cat : 13520-1-AP

Synonymes

CHETK alpha, CHK, CHKA, Choline kinase alpha, CK, CKI, CK-α, EK, Ethanolamine kinase;

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Galerie de données de validation

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  WB analysis using 13520-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13520-1-AP (Choline kinase alpha antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of COLO 320 using 13520-1-AP

  COLO 320 cells were subjected to SDS PAGE followed by western blot with 13520-1-AP (Choline kinase alpha antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse colon using 13520-1-AP

  mouse colon tissue were subjected to SDS PAGE followed by western blot with 13520-1-AP (Choline kinase alpha antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse spleen using 13520-1-AP

  IP Result of anti-Choline kinase alpha (IP:13520-1-AP, 4ug; Detection:13520-1-AP 1:500) with mouse spleen tissue lysate 4000ug.

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  IHC staining of human liver cancer using 13520-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13520-1-AP (Choline kinase alpha antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of human liver cancer using 13520-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13520-1-AP (Choline kinase alpha antibody) at dilution of 1:200 (under 40x lens).

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  IF Staining of HeLa using 13520-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using 13520-1-AP (Choline kinase alpha antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HeLa using 13520-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 13520-1-AP (Choline kinase alpha antibody), at dilution of 1:100 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu splénique de souris, cellules COLO 320, tissu de côlon de souris, tissu splénique de rat
• Résultats positifs en IP: tissu splénique de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 18 publications below
• IHC: See 5 publications below
• IF: See 2 publications below
• ELISA: See 1 publications below

Informations sur le produit

13520-1-AP cible Choline Kinase Alpha dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Choline Kinase Alpha Protéine recombinante Ag4761
• Nom complet: choline kinase alpha
• Masse moléculaire calculée: 439 aa, 52 kDa
• Poids moléculaire observé: 50 kDa
• Numéro d’acquisition GenBank: BC036471
• Symbole du gène: CHKA
• Identification du gène (NCBI): 1119
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CHKA(Choline kinase alpha) is also named as CHK, CKI, EK(Ethanolamine kinase) and belongs to the choline/ethanolamine kinase family. Choline kinase (ATP:choline phosphotransferase) is a cytosolic enzyme that catalyzes the committed step in the synthesis of PC by the CDP-choline pathway. The enzyme catalyzes the phosphorylation of choline with ATP to form phosphocholine and ADP(PMID:9506987). It has 2 isoforms produced by alternative splicing with the molecular weight of 52 kDa and 50 kDa.

Publications

Species	Application	Title
human	WB,IHC	Proc Natl Acad Sci U S A; The moonlighting function of glycolytic enzyme enolase-1 promotes choline phospholipid metabolism and tumor cell proliferation; Authors - Qingxia Ma
mouse	WB, IHC	Ann Rheum Dis; Choline kinase inhibition in rheumatoid arthritis.; Authors - M Guma
human	WB	Clin Cancer Res; Preclinical development of a non-toxic oral formulation of monoethanolamine, a lipid precursor, for prostate cancer treatment.; Authors - Roopali Saxena
mouse	WB	Front Cell Dev Biol; Choline Kinase Alpha Is a Novel Transcriptional Target of the Brg1 in Hepatocyte: Implication in Liver Regeneration.; Authors - Ming Kong
rat	WB	J Biol Chem; ras-Induced up-regulation of CTP:phosphocholine cytidylyltransferase α contributes to malignant transformation of intestinal epithelial cells.; Authors - Arsenault Daniel J DJ
	WB	Mol Biol Cell; Nuclear-localized CTP:phosphocholine cytidylyltransferase α regulates phosphatidylcholine synthesis required for lipid droplet biogenesis.; Authors - Adam J Aitchison

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Hua (Verified Customer) (02-02-2021)

I was surprised it did not work as shown in the datasheet. It is possible jus this batch?

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: mouse liver and mouse spleen

Choline Kinase Alpha Antibody Western Blot validation (1:500 dilution) in mouse liver and mouse spleen (Cat no:13520-1-AP)