Anticorps Polyclonal de lapin anti-CHMP1A

• Phare
• Validé par KD/KO

CHMP1A Polyclonal Antibody for IF, IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

8

N° de cat : 15761-1-AP

Synonymes

CHMP1, CHMP1A, chromatin modifying protein 1A, hVps46 1, KIAA0047, PCOLN3, PRSM1, Vps46 1

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Galerie de données de validation

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  WB analysis of HEK-293 using 15761-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 15761-1-AP (CHMP1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 15761-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 15761-1-AP (CHMP1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse lung using 15761-1-AP

  mouse lung tissue were subjected to SDS PAGE followed by western blot with 15761-1-AP (CHMP1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A431 using 15761-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 15761-1-AP (CHMP1A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse kidney using 15761-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 15761-1-AP (CHMP1A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 15761-1-AP

  IP Result of anti-CHMP1A (IP:15761-1-AP, 3ug; Detection:15761-1-AP 1:500) with HEK-293 cells lysate 880ug.

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  IHC staining of mouse brain using 15761-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15761-1-AP (CHMP1A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse brain using 15761-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 15761-1-AP (CHMP1A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse testis using 15761-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15761-1-AP (CHMP1A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse testis using 15761-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse testis tissue slide using 15761-1-AP (CHMP1A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 15761-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 15761-1-AP (CHMP1A antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human lung using 15761-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung tissue slide using 15761-1-AP (CHMP1A antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skin using 15761-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15761-1-AP (CHMP1A antibody) at dilution of 1:100 (under 10x lens).

• 

  IHC staining of human skin using 15761-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 15761-1-AP (CHMP1A antibody) at dilution of 1:100 (under 40x lens).

• 

  IF Staining of HepG2 using 15761-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed HepG2 cells using 15761-1-AP (CHMP1A antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules A431, cellules HeLa, tissu pulmonaire de souris, tissu rénal de souris
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu cérébral de souris, tissu cutané humain, tissu pulmonaire humain, tissu testiculaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 5 publications below
• IHC: See 1 publications below
• IF: See 3 publications below

Informations sur le produit

15761-1-AP cible CHMP1A dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CHMP1A Protéine recombinante Ag8389
• Nom complet: chromatin modifying protein 1A
• Masse moléculaire calculée: 196 aa, 22 kDa
• Poids moléculaire observé: 33 kDa, 25 kDa
• Numéro d’acquisition GenBank: BC007527
• Symbole du gène: CHMP1A
• Identification du gène (NCBI): 5119
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Charged multivesicular body protein 1A (CHMP1A), also known as chromatin modifying protein 1A, is a member of the ESCRT-III (endosomal sorting complex required for transport III) complex which is involved in degradation of internalized transmembrane receptor proteins and formation of multivesicular bodies (MVBs) (PMID: 16730941). CHMP1A has both nuclear and cytoplasmic distributions (PMID: 11559748; 11559747). Subcellular localization of CHMP1A seems to vary depending on the cell type (PMID: 23023333). CHMP1A is required for MVBs formation and has a role in stable gene silencing within the nucleus. Besides, the tumor suppressor role of CHMP1A has also been reported (PMID: 18787405; 22261332).

Publications

Species	Application	Title
human	IF	Nat Cell Biol; Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.; Authors - Vincent Mercier
human	WB	J Extracell Vesicles; Accessory ESCRT-III proteins are conserved and selective regulators of Rab11a-exosome formation; Authors - Pauline P Marie
mouse	WB,IF	Nat Commun; A single genetic locus controls both expression of DPEP1/CHMP1A and kidney disease development via ferroptosis.; Authors - Yuting Guan ; KO Validated
mouse	WB	Nat Commun; Mechanism of Ca²⁺-triggered ESCRT assembly and regulation of cell membrane repair.; Authors - Luana L Scheffer
human,mouse	IHC	Cell Rep; The ESCRT-III Protein CHMP1A Mediates Secretion of Sonic Hedgehog on a Distinctive Subtype of Extracellular Vesicles.; Authors - Michael E Coulter ; KO Validated
human	WB	Cell Rep; Resolving ESCRT-III Spirals at the Intercellular Bridge of Dividing Cells Using 3D STORM.; Authors - Inna Goliand ; KD Validated

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