Anticorps Polyclonal de lapin anti-CHMP4B

• Phare
• Validé par KD/KO

CHMP4B Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (2)

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

48

Avis (1)

N° de cat : 13683-1-AP

Synonymes

C20orf178, CHMP4B, chromatin modifying protein 4B, CTPP3, hSnf7 2, hVps32 2, Shax1, SNF7, SNF7 2, Vps32 2

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Galerie de données de validation

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  WB analysis of mouse heart using 13683-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13683-1-AP (CHMP4B antibody) at dilution of 1:200 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 13683-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 13683-1-AP (CHMP4B Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 13683-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 13683-1-AP (CHMP4B Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human oesophagus cancer using 13683-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer using 13683-1-AP (CHMP4B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human oesophagus cancer using 13683-1-AP

  Immunohistochemical analysis of paraffin-embedded human oesophagus cancer using 13683-1-AP (CHMP4B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 13683-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13683-1-AP (CHMP4B antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human heart using 13683-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 13683-1-AP (CHMP4B antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human skeletal muscle using 13683-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 13683-1-AP (CHMP4B Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 13683-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 13683-1-AP (CHMP4B Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 13683-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CHMP4B antibody (13683-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HepG2 using 13683-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CHMP4B antibody (13683-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of NIH/3T3 using 13683-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed NIH/3T3 cells using CHMP4B antibody (13683-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of H9C2 using 13683-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed H9C2 cells using CHMP4B antibody (13683-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of C2C12 using 13683-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed C2C12 cells using CHMP4B antibody (13683-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, cellules HeLa, tissu cardiaque de rat
• Résultats positifs en IHC: tissu de cancer de l'œsophage humain, tissu cardiaque humain, tissu de muscle squelettique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules NIH/3T3, cellules C2C12, cellules H9C2, cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 7 publications below
• WB: See 18 publications below
• IF: See 33 publications below
• IP: See 3 publications below
• CoIP: See 1 publications below

Informations sur le produit

13683-1-AP cible CHMP4B dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, canin, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CHMP4B Protéine recombinante Ag4544
• Nom complet: chromatin modifying protein 4B
• Masse moléculaire calculée: 224 aa, 25 kDa
• Poids moléculaire observé: 25-33 kDa
• Numéro d’acquisition GenBank: BC033859
• Symbole du gène: CHMP4B
• Identification du gène (NCBI): 128866
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CHMP4B (charged multivesicular body protein 4b) is a human homologue of yeast Snf7p (also named Vps32p) and a member of CHMP family which is implicated in multivesicular body (MVB) sorting. The CHMP proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies. Among seven CHMP family members, only CHMP4 has been found to interact with Alix, a multifunctional adaptor protein in various cellular functions. This antibody is highly specific to the CHMP4B and it doesn't cross-react with other CHMPs as well as other CHMP4s (CHMP4A and CHMP4C). CHMP4B can be detected 25-33 kDa by post-translational modification (PMID:26490837).

Publications

Species	Application	Title
human	IF	Nature; Nuclear envelope assembly defects link mitotic errors to chromothripsis.; Authors - Shiwei Liu
human	IF	Cell; Mammalian hybrid pre-autophagosomal structure HyPAS generates autophagosomes; Authors - Suresh Kumar
human	IF	Nat Cell Biol; Endosomal membrane tension regulates ESCRT-III-dependent intra-lumenal vesicle formation.; Authors - Vincent Mercier
		Neuron; Brain-derived autophagosome profiling reveals the engulfment of nucleoid-enriched mitochondrial fragments by basal autophagy in neurons.; Authors - Juliet Goldsmith
human	CoIP	Autophagy; Periplocin suppresses the growth of colorectal cancer cells by triggering LGALS3 (galectin 3)-mediated lysophagy; Authors - Kui Wang
human	IF	Sci Adv; Caveolae promote successful abscission by controlling intercellular bridge tension during cytokinesis.; Authors - Virginia Andrade

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Sharad (Verified Customer) (08-30-2019)

The antibody works well in IF staining 1:500 dilution.

• Applications: Immunofluorescence,
• Cell Tissue Type: RPE-1

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