Anticorps Recombinant de lapin anti-CHMP4B

CHMP4B Recombinant Antibody for WB, FC (Intra)

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, FC (Intra)

Conjugaison

CoraLite® Plus 750 Fluorescent Dye

CloneNo.

2N22

N° de cat : CL750-81691-2

Synonymes

hVps32-2, hSnf7-2, Chromatin-modifying protein 4b, chromatin modifying protein 4B, C20orf178

---

Galerie de données de validation

• 

  WB analysis using CL750-81691-2

  Various lysates were subjected to SDS PAGE followed by western blot with CL750-81691-2 (CHMP4B antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

• 

  FC experiment of HepG2 using CL750-81691-2

  1x10^6 HepG2 cells were intracellularly stained with 0.4 ug CoraLite® Plus 750-conjugated CHMP4B Recombinant antibody (CL750-81691-2, Clone:2N22)(red), or 0.4 ug Isotype Control (CL750-30000) (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

---

Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules HuH-7
• Résultats positifs en FC (Intra): cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.40 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

CL750-81691-2 cible CHMP4B dans les applications de WB, FC (Intra) et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Recombinant
• Type: Anticorps
• Immunogène: CHMP4B Protéine recombinante Ag4544
• Nom complet: chromatin modifying protein 4B
• Masse moléculaire calculée: 224 aa, 25 kDa
• Poids moléculaire observé: 25 kDa
• Numéro d’acquisition GenBank: BC033859
• Symbole du gène: CHMP4B
• Identification du gène (NCBI): 128866
• Conjugaison: CoraLite® Plus 750 Fluorescent Dye
• Excitation/Emission maxima wavelengths: 755 nm / 780 nm
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 50% glycerol, 0.05% Proclin300, 0.5% BSA
• Conditions de stockage: Stocker à -20 °C. Éviter toute exposition à la lumière. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CHMP4B (charged multivesicular body protein 4b) is a human homologue of yeast Snf7p (also named Vps32p) and a member of CHMP family which is implicated in multivesicular body (MVB) sorting. The CHMP proteins are components of ESCRT-III (endosomal sorting complex required for transport III), a complex involved in degradation of surface receptor proteins and formation of endocytic multivesicular bodies. Among seven CHMP family members, only CHMP4 has been found to interact with Alix, a multifunctional adaptor protein in various cellular functions. This antibody is highly specific to the CHMP4B and it doesn't cross-react with other CHMPs as well as other CHMP4s (CHMP4A and CHMP4C). CHMP4B can be detected 25-33 kDa by post-translational modification (PMID:26490837).

{{ptg:RelatedPrimaryAntibodies}}