Anticorps Polyclonal de lapin anti-CHMP7

• Phare
• Validé par KD/KO

CHMP7 Polyclonal Antibody for IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

8

Avis (1)

N° de cat : 16424-1-AP

Synonymes

CHMP family, member 7, CHMP7, Chromatin modifying protein 7

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Galerie de données de validation

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  WB analysis using 16424-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16424-1-AP (CHMP7 antibody) at dilution of 1:2500 incubated at room temperature for 1.5 hours.

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  WB analysis using 16424-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16424-1-AP (CHMP7 antibody) at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse spinal cord using 16424-1-AP

  mouse spinal cord tissue were subjected to SDS PAGE followed by western blot with 16424-1-AP (CHMP7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of rat spinal cord using 16424-1-AP

  rat spinal cord tissue were subjected to SDS PAGE followed by western blot with 16424-1-AP (CHMP7 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human kidney using 16424-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16424-1-AP (CHMP7 antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human kidney using 16424-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 16424-1-AP (CHMP7 antibody) at dilution of 1:100 (under 40x lens).

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  IF Staining of A431 using 16424-1-AP

  Immunofluorescent analysis of (4% PFA) fixed A431 cells using CHMP7 antibody (16424-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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  IF Staining of A431 using 16424-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed A431 cells using CHMP7 antibody (16424-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HEK-293T, cellules HeLa, cellules Jurkat, tissu de moelle épinière de rat, tissu de moelle épinière de souris
• Résultats positifs en IHC: tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules A431,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:16000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 5 publications below
• WB: See 7 publications below
• IHC: See 1 publications below
• IF: See 2 publications below
• IP: See 2 publications below

Informations sur le produit

16424-1-AP cible CHMP7 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, porc
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CHMP7 Protéine recombinante Ag9625
• Nom complet: CHMP family, member 7
• Masse moléculaire calculée: 453 aa, 51 kDa
• Poids moléculaire observé: 51-55 kDa
• Numéro d’acquisition GenBank: BC019110
• Symbole du gène: CHMP7
• Identification du gène (NCBI): 91782
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB,IF	Nat Cell Biol; Unrestrained ESCRT-III drives micronuclear catastrophe and chromosome fragmentation.; Authors - Marina Vietri ; KD Validated
human	WB	Dev Cell; CC2D1B Coordinates ESCRT-III Activity during the Mitotic Reformation of the Nuclear Envelope.; Authors - Leandro N Ventimiglia ; KD Validated
human	WB	Dev Cell; The ESCRT machinery counteracts Nesprin-2G-mediated mechanical forces during nuclear envelope repair.; Authors - Samuel S Wallis ; KD Validated
human	WB	Curr Biol; Membrane Binding by CHMP7 Coordinates ESCRT-III-Dependent Nuclear Envelope Reformation.; Authors - Yolanda Olmos ; KD Validated
human	WB,IP	Elife; CDK1 controls CHMP7-dependent nuclear envelope reformation.; Authors - Alberto T Gatta ; KD Validated
pig	WB,IF,IP	PLoS Pathog; Cellular ESCRT components are recruited to regulate the endocytic trafficking and RNA replication compartment assembly during classical swine fever virus infection.; Authors - Chun-Chun Liu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Viviana (Verified Customer) (06-01-2022)

Very good to IP endogenous protein from mouse tissue (2 ug for less than 2mg of total protein tested) and also for western blot.

• Applications: Western Blot, Immunoprecipitation
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: mouse embryonic tissue

CHMP7 Antibody Western Blot,Immunoprecipitation validation (1:1000 dilution) in mouse embryonic tissue (Cat no:16424-1-AP)