Anticorps Polyclonal de lapin anti-CHRNA1

CHRNA1 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(13)

N° de cat : 10613-1-AP

Synonymes

CHNRA, ACHRA

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Galerie de données de validation

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  WB analysis of mouse heart using 10613-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 10613-1-AP (CHRNA1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat heart using 10613-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 10613-1-AP (CHRNA1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  IHC staining of mouse skeletal muscle using 10613-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 10613-1-AP (CHRNA1 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse skeletal muscle using 10613-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse skeletal muscle tissue slide using 10613-1-AP (CHRNA1 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human skeletal muscle using 10613-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 10613-1-AP (CHRNA1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human skeletal muscle using 10613-1-AP

  Immunohistochemical analysis of paraffin-embedded human skeletal muscle tissue slide using 10613-1-AP (CHRNA1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 10613-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10613-1-AP (CHRNA1 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human heart using 10613-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 10613-1-AP (CHRNA1 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IF Staining of C2C12 using 10613-1-AP

  Immunofluorescent analysis of (-20℃ Ethanol) fixed C2C12 cell using 10613-1-AP (CHRNA1 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, tissu cardiaque de rat
• Résultats positifs en IHC: tissu de muscle squelettique de souris, tissu cardiaque humain, tissu de muscle squelettique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellule C2C12

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 10 publications below
• IHC: See 3 publications below

Informations sur le produit

10613-1-AP cible CHRNA1 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CHRNA1 Protéine recombinante Ag0933
• Nom complet: cholinergic receptor, nicotinic, alpha 1 (muscle)
• Masse moléculaire calculée: 54.5 kDa
• Poids moléculaire observé: 54 kDa
• Numéro d’acquisition GenBank: BC006314
• Symbole du gène: CHRNA1
• Identification du gène (NCBI): 1134
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	WB	Aging (Albany NY); A five-m6A regulatory gene signature is a prognostic biomarker in lung adenocarcinoma patients.; Authors - Xiao Wu
mouse	WB	Aging (Albany NY); Maternal high sugar and fat diet benefits offspring brain function via targeting on the gut-brain axis.; Authors - Dongdong Wang
mouse	WB	Transl Psychiatry; A diet high in sugar and fat influences neurotransmitter metabolism and then affects brain function by altering the gut microbiota.; Authors - Yinrui Guo
human	WB	Sci Rep; Liprin-α-1 is a novel component of the murine neuromuscular junction and is involved in the organization of the postsynaptic machinery.; Authors - Krzysztof M Bernadzki
rat	WB	Front Pharmacol; Miya Improves Osteoarthritis Characteristics via the Gut-Muscle-Joint Axis According to Multi-Omics Analyses.; Authors - Tianyang Xu
mouse	WB	Sci Rep; An improved method for culturing myotubes on laminins for the robust clustering of postsynaptic machinery.; Authors - Marcin Pęziński

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