Anticorps Polyclonal de lapin anti-CHST15

• Phare
• Validé par KD/KO

CHST15 Polyclonal Antibody for IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 14298-1-AP

Synonymes

BRAG, CHST15, GALNAC4S 6ST, GALNAC4S6ST, hBRAG, KIAA0598, RP11 47G11.1

---

Galerie de données de validation

• 

  WB analysis using 14298-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14298-1-AP (CHST15 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse spleen using 14298-1-AP

  mouse spleen tissue were subjected to SDS PAGE followed by western blot with 14298-1-AP (CHST15 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IP experiment of Raji using 14298-1-AP

  IP Result of anti-CHST15 (IP:14298-1-AP, 4ug; Detection:14298-1-AP 1:500) with Raji cells lysate 3200ug.

---

Applications testées

• Résultats positifs en WB: cellules Jurkat, cellules Raji, cellules U-937, tissu cardiaque de rat, tissu cardiaque de souris, tissu splénique de souris
• Résultats positifs en IP: cellules Raji

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

14298-1-AP cible CHST15 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CHST15 Protéine recombinante Ag5679
• Nom complet: carbohydrate (N-acetylgalactosamine 4-sulfate 6-O) sulfotransferase 15
• Masse moléculaire calculée: 65 kDa
• Poids moléculaire observé: 49 kDa, 69 kDa
• Numéro d’acquisition GenBank: BC050540
• Symbole du gène: CHST15
• Identification du gène (NCBI): 51363
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Carbohydrate sulfotransferase 15, also known as CHST15, BRAG, GALNAC4S6ST and KIAA0598, is a single-pass type II membrane protein which belongs to the sulfotransferase 1 family. CHST15 is a sulfotransferase that transfers sulfate from 3'-phosphoadenosine 5'-phosphosulfate (PAPS) to the C-6 hydroxyl group of the GalNAc 4-sulfate residue of chondroitin sulfate A and forms chondroitin sulfate E containing GlcA-GalNAc(4,6-SO4) repeating units. It also transfers sulfate to a unique non-reducing terminal sequence, GalNAc(4SO4)-GlcA(2SO4)-GalNAc(6SO4), to yield a highly sulfated structure similar to the structure found in thrombomodulin chondroitin sulfate. CHST15 may also act as a B-cell receptor involved in BCR ligation-mediated early activation that mediate regulatory signals key to B-cell development and / or regulation of B-cell-specific RAG expression. CHST15 is expressed in B-cell-enriched tissues but not in fetal or adult thymus. It is expressed in fetal and adult spleen, lymph node, tonsil, bone marrow and peripheral leukocytes. It can be detected as 49 kDa and 69 kDa.

Publications

Species	Application	Title
mouse	WB	Elife; Chondroitin sulfate proteoglycan 4,6 sulfation regulates sympathetic nerve regeneration after myocardial infarction.; Authors - Matthew R Blake ; KD Validated
human	IHC	Osteoarthritis Cartilage; Changes in the metabolism of chondroitin sulfate glycosaminoglycans in articular cartilage from patients with Kashin-Beck Disease.; Authors - Mingxiu Luo
human	IF	Ann Transl Med; Transcriptome analysis of tumor-derived mesenchymal progenitor cells shows that CHST15 is a fibrosis regulator of retroperitoneal liposarcoma.; Authors - Yang Sun
mouse	WB	Physiol Rep; Loss of chondroitin sulfate proteoglycan sulfation allows delayed sympathetic reinnervation after cardiac ischemia-reperfusion; Authors - Matthew R Blake