Anticorps Polyclonal de lapin anti-CIDEA

CIDEA Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

15

N° de cat : 13170-1-AP

Synonymes

Cell death activator CIDE A, CIDE A, CIDEA

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Galerie de données de validation

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  WB analysis of mouse heart using 13170-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13170-1-AP (CIDEA antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 13170-1-AP

  Mouse heart tissue was subjected to SDS PAGE followed by western blot with 13170-1-AP (CIDEA antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brown adipose using 13170-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 13170-1-AP (CIDEA antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brown adipose using 13170-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brown adipose tissue slide using 13170-1-AP (CIDEA antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris,
• Résultats positifs en IHC: tissu adipeux brun de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 14 publications below
• IHC: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

13170-1-AP cible CIDEA dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CIDEA Protéine recombinante Ag3824
• Nom complet: cell death-inducing DFFA-like effector a
• Masse moléculaire calculée: 253 aa, 28 kDa
• Poids moléculaire observé: 25 kDa
• Numéro d’acquisition GenBank: BC031896
• Symbole du gène: CIDEA
• Identification du gène (NCBI): 1149
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cell death-inducing DNA fragmentation factor (DFFA)-like effector (CIDE) family proteins, including Cidea, Cideb, and Fsp27 (Cidec), are lipid droplet (LD)-associated proteins playing important roles in LD fusion, lipid secretion, and very-low-density-lipoprotein (VLDL) maturation. Cidea is highly enriched in adult brown adipose tissue (BAT), and its deficiency results in the accumulation of smaller LDs in brown adipocytes, improved INS sensitivity, and resistance to diet-induced obesity. The MW of this protein is 25 kDa, and this antibody specially recognises the 25 kDa protein.

Publications

Species	Application	Title
mouse	WB	Nature; CLSTN3β enforces adipocyte multilocularity to facilitate lipid utilization; Authors - Kevin Qian
mouse	IF	Nat Commun; Dynamic enlargement and mobilization of lipid droplets in pluripotent cells coordinate morphogenesis during mouse peri-implantation development.; Authors - King Hang Tommy Mau
mouse	WB	EMBO J; Asparagine reinforces mTORC1 signaling to boost thermogenesis and glycolysis in adipose tissues.; Authors - Yingjiang Xu
mouse	WB	Diabetes; Fat-Specific Knockout of Mecp2 Upregulates Slpi to Reduce Obesity by Enhancing Browning.; Authors - Chengyu Liu
mouse	WB,IHC	J Agric Food Chem; Polysaccharide from Ziyang Selenium-Enriched Green Tea Prevents Obesity and Promotes Adipose Thermogenesis via Modulating the Gut Microbiota; Authors - Donglu Li
mouse	WB,IHC	Food Funct; Artemisia sphaerocephala Krasch polysaccharide promotes adipose thermogenesis and decreases obesity by shaping the gut microbiota; Authors - Xiaoqian Zeng