Anticorps Polyclonal de lapin anti-CIDEC

CIDEC Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 12287-1-AP

Synonymes

Cell death activator CIDE 3, CIDE 3, CIDEC, Fsp27

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Galerie de données de validation

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  WB analysis of MCF-7 using 12287-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 12287-1-AP (CIDEC Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of L02 using 12287-1-AP

  L02 cells were subjected to SDS PAGE followed by western blot with 12287-1-AP (CIDEC Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules MCF-7, cellules L02

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

12287-1-AP cible CIDEC dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CIDEC Protéine recombinante Ag2913
• Nom complet: cell death-inducing DFFA-like effector c
• Masse moléculaire calculée: 238 aa, 27 kDa
• Poids moléculaire observé: 27-35 kDa
• Numéro d’acquisition GenBank: BC016851
• Symbole du gène: CIDEC
• Identification du gène (NCBI): 63924
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Cell death-inducing DFF45-like effector (CIDE) proteins, including CIDEA, CIDEB and CIDEC (also called Fsp27 in rodents), are predominantly expressed in brown adipose tissues (BAT), liver and WAT. CIDEC was found to be highly expressed in adipose tissues and to function as a lipid droplet-binding protein that promotes lipid accumulation in adipocytes. While normal mouse livers express low levels of CIDEC protein, its expression is highly upregulated in NALFD, but not in chronic ethanol feeding-induced fatty liver. (PMID: 26099526) Catalog#12287-1-AP recognizing CIDEC (calculated 28 kDa) as a 30 kDa and 45-50 kDa due to its posttranslational modification (PMID: 19258494).

Publications

Species	Application	Title
human	WB	Commun Biol; Glutathione S-transferase Mu 2 inhibits hepatic steatosis via ASK1 suppression.; Authors - Yi Jin
human	WB	Biomed Pharmacother; Dihydroartemisinin inhibits ER stress-mediated mitochondrial pathway to attenuate hepatocyte lipoapoptosis via blocking the activation of the PI3K/Akt pathway.; Authors - Xingran Chen
mouse	WB	Aging Cell; Long-term NAD+ supplementation prevents the progression of age-related hearing loss in mice; Authors - Mustafa N Okur