Anticorps Polyclonal de lapin anti-CILP2

CILP2 Polyclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 11813-1-AP

Synonymes

CILP 2, CILP2, CLIP 2

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Galerie de données de validation

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  WB analysis of K-562 using 11813-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11813-1-AP (CILP2 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 11813-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11813-1-AP (CILP2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 11813-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11813-1-AP (CILP2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse brain using 11813-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 11813-1-AP (CILP2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of K-562 using 11813-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 11813-1-AP (CILP2 antibody) at dilution of 1:100 incubated at room temperature for 1.5 hours.

• 

  IF Staining of HEK-293 using 11813-1-AP

  Immunofluorescent analysis of HEK-293 cells using 11813-1-AP (CILP2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules K-562, cellules HEK-293, tissu cérébral de souris
• Résultats positifs en IF: cellules HEK-293

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunofluorescence (IF): IF : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

11813-1-AP cible CILP2 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CILP2 Protéine recombinante Ag2366
• Nom complet: cartilage intermediate layer protein 2
• Masse moléculaire calculée: 126 kDa
• Poids moléculaire observé: 90 kDa
• Numéro d’acquisition GenBank: BC034926
• Symbole du gène: CILP2
• Identification du gène (NCBI): 148113
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CILP2, also named as Cartilage intermediate layer protein 2, is a 1156 amino acid protein, which is expressed in articular chondrocytes but not in knee meniscal cartilage cells. CILP2 localizes to the intermediate to deep zone of articular cartilage. CILP2 may play a role in cartilage scaffolding. Given the full-length molecular mass of CILP2 is 125 kDa, but the most CILP2 protein in articular cartilage is cleaved,mostly likely at the furin cleavage site. CILP2 protein is detected as a major band on SDS-page migrating at about 90 kDa. (PMID: 21880736)

Publications

Species	Application	Title
mouse	WB	Cell Rep; Endothelial cells regulated by RNF20 orchestrate the proliferation and differentiation of neural precursor cells during embryonic development; Authors - Mengtian Zhang