Anticorps Polyclonal de lapin anti-CIN85

• Phare
• Validé par KD/KO

CIN85 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(3)

N° de cat : 12132-1-AP

Synonymes

SH3KBP1, HSB-1, HSB 1, CD2BP3, CD2-binding protein 3

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Galerie de données de validation

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  WB analysis using 12132-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 12132-1-AP (CIN85 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of fetal human brain using 12132-1-AP

  fetal human brain tissue were subjected to SDS PAGE followed by western blot with 12132-1-AP (CIN85 Antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IHC staining of human colon cancer using 12132-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12132-1-AP (CIN85 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human colon cancer using 12132-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 12132-1-AP (CIN85 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary cancer using 12132-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 12132-1-AP (CIN85 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules Raji, cellules Jurkat, cellules NIH/3T3, cellules Ramos, cellules THP-1, tissu cérébral humain fœtal
• Résultats positifs en IHC: tissu de cancer du côlon humain, human ovary cancer tissue; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

12132-1-AP cible CIN85 dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CIN85 Protéine recombinante Ag2777
• Nom complet: SH3-domain kinase binding protein 1
• Masse moléculaire calculée: 665 aa, 73 kDa
• Poids moléculaire observé: 85 kDa
• Numéro d’acquisition GenBank: BC015806
• Symbole du gène: SH3KBP1/CIN85
• Identification du gène (NCBI): 30011
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CIN85 is identified as a Cbl-interacting protein of 85 kDa and belongs to adaptor/scaffold proteins. It composed of three N-terminal SH3 domains followed by a proline-rich region and a C-terminal coiled-coil region. In association with c-Cbl (Casitas B-lineage lymphoma), an E3 ubiquitin ligase. As an adaptor protein, CIN85 is involved in Cbl-dependent EGFR internalization, intracellular receptor trafficking, sorting and degradation. Moreover, through the SH3 domains and the proline-rich region, CIN85 is implicated in many protein-protein interactions and it is found to play important roles in other processes such as apoptosis, rearrangement of actin cytoskeleton, cell adhesion, immunological synapse, cell migration and invasion.

Publications

Species	Application	Title
human	WB, IF	J Cell Biol; CIN85 modulates TGFβ signaling by promoting the presentation of TGFβ receptors on the cell surface.; Authors - Ihor Yakymovych
human	WB	Biol Cell; ARAP1 association with CIN85 affects epidermal growth factor receptor endocytic trafficking.; Authors - Yoon Hye-Young HY ; KD Validated
human	IHC	Arch Gynecol Obstet; Significance of PTPRZ1 and CIN85 expression in cervical carcinoma.; Authors - Ma Yaxi Y

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