Anticorps Polyclonal de lapin anti-CISD2

• Phare
• Validé par KD/KO

CISD2 Polyclonal Antibody for FC, IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IP, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

21

N° de cat : 13318-1-AP

Synonymes

CDGSH iron sulfur domain 2, CDGSH2, CISD2, ERIS, Miner1, MitoNEET related 1 protein, NAF 1, WFS2, ZCD2

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Galerie de données de validation

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  WB analysis using 13318-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13318-1-AP (CISD2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse heart using 13318-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 13318-1-AP (CISD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 13318-1-AP

  human brain tissue were subjected to SDS PAGE followed by western blot with 13318-1-AP (CISD2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 13318-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 13318-1-AP (CISD2 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse brain using 13318-1-AP

  IP Result of anti-CISD2 (IP:13318-1-AP, 3ug; Detection:13318-1-AP 1:700) with mouse brain tissue lysate 4000ug.

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  IHC staining of human liver cancer using 13318-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13318-1-AP (CISD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 13318-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 13318-1-AP (CISD2 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 13318-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CISD2 antibody (13318-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 13318-1-AP

  Immunofluorescent analysis of HepG2 cells using 13318-1-AP (CISD2 antibody) at dilution of 1:50 and Rhodamine-Goat anti-Rabbit IgG.

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  FC experiment of HepG2 using 13318-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug CISD2 antibody (13318-1-AP, red) and control antibody (blue). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1500. Cells were fixed with 4% PFA and permeabilized with 0.1% Triton X-100.

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Applications testées

• Résultats positifs en WB: tissu rénal de souris, tissu cardiaque de souris, tissu cérébral de souris, tissu cérébral humain, tissu rénal de rat
• Résultats positifs en IP: tissu cérébral de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:200-1:800
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 8 publications below
• WB: See 20 publications below
• IHC: See 10 publications below
• IF: See 4 publications below

Informations sur le produit

13318-1-AP cible CISD2 dans les applications de WB, IP, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Drosophile, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CISD2 Protéine recombinante Ag4172
• Nom complet: CDGSH iron sulfur domain 2
• Masse moléculaire calculée: 135 aa, 15 kDa
• Poids moléculaire observé: 13-15 kDa
• Numéro d’acquisition GenBank: BC032300
• Symbole du gène: CISD2
• Identification du gène (NCBI): 493856
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CISD2 gene encodes a 15 kDa CDGSH iron-sulfur domain-containing protein 2, which is also named Miner1 or NAF-1, this protein was reported on endoplasmic reticulum membrane or mitochondrion outer membrane. Defects in CISD2 are the cause of Wolfram syndrome type 2 (WFS2), a rare disorder characterized by juvenile-onset insulin-dependent diabetes mellitus with optic atrophy. CISD2 regulates autophagy program by interacting BCL2, contributing to antagonize BECN1-mediated cellular autophagy at the endoplasmic reticulum.

Publications

Species	Application	Title
human, mouse, rat	WB	Proc Natl Acad Sci U S A; A calcium-dependent protease as a potential therapeutic target for Wolfram syndrome.; Authors - Simin Lu ; KD Validated
	WB	Elife; Sampling the conformational space of the catalytic subunit of human γ-secretase.; Authors - Xiao-Chen Bai
human	WB	EMBO Rep; Chemical targeting of NEET proteins reveals their function in mitochondrial morphodynamics.; Authors - Diana Molino ; KD Validated
human	WB,IHC	Oncotarget; Overexpressed CISD2 has prognostic value in human gastric cancer and promotes gastric cancer cell proliferation and tumorigenesis via AKT signaling pathway.; Authors - Lan Wang ; KD Validated
mouse	WB,IHC	FASEB J; A young testicular microenvironment protects Leydig cells against age-related dysfunction in a mouse model of premature aging.; Authors - Michael Curley ; KO Validated
human	WB,IHC	Oncotarget; A novel prognostic score model incorporating CDGSH Iron Sulfur Domain2 (CISD2) predicts risk of disease progression in laryngeal squamous cell carcinoma.; Authors - Lin Yang

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