Anticorps Polyclonal de lapin anti-CIT

CIT Polyclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 20033-1-AP

Synonymes

CIT, Citron Rho interacting kinase, CRIK, KIAA0949, STK21

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Galerie de données de validation

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  WB analysis of A431 using 20033-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 20033-1-AP (CIT antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  WB analysis using 20033-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 20033-1-AP (CIT antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 20033-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 20033-1-AP (CIT antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 20033-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 20033-1-AP (CIT antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IF Staining of HepG2 using 20033-1-AP

  Immunofluorescent analysis of HepG2 cells, using CIT antibody 20033-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HeLa, tissu cérébral de rat, tissu cérébral de souris
• Résultats positifs en IF: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

20033-1-AP cible CIT dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: citron (rho-interacting, serine/threonine kinase 21)
• Masse moléculaire calculée: 231 kDa
• Poids moléculaire observé: 230 kDa, 177 kDa
• Numéro d’acquisition GenBank: NM_007174
• Symbole du gène: CIT
• Identification du gène (NCBI): 11113
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CIT, also named KIAA0949, STK21 and CRIK, belongs to the protein kinase superfamily and AGC Ser/Thr protein kinase family. It is required for KIF14 localization to the central spindle and midbody. CIT may play a role in cytokinesis. Putative RHO/RAC effector that binds to the GTP-bound forms of RHO and RAC1. It probably binds p21 with a tighter specificity in vivo. CIT plays an important role in the regulation of cytokinesis and the development of the central nervous system. The antibody recognizes all the isoforms of CIT.

Publications

Species	Application	Title
human	WB	Int J Mol Sci; Identification of Overexpressed Genes in Malignant Pleural Mesothelioma.; Authors - Federica Morani
mouse	WB	Mol Cell Biol; E2F-2 Promotes Nuclear Condensation and Enucleation of Terminally Differentiated Erythroblasts.; Authors - Kelsey L Swartz
human	WB	Sci Rep; A three-gene signature reveals changes in the tumor immune microenvironment in the progression from NAFLD to HCC; Authors - Lijuan Liu