Anticorps Polyclonal de lapin anti-CITED2

CITED2 Polyclonal Antibody for IF, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF, ELISA

Conjugaison

Non conjugué

Publications

1

N° de cat : 15021-1-AP

Synonymes

CITED2, MRG 1, MRG1, MSG related protein 1, P35SRJ

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Galerie de données de validation

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  WB analysis of SH-SY5Y using 15021-1-AP

  SH-SY5Y cells were subjected to SDS PAGE followed by western blot with 15021-1-AP (CITED2 antibody) at dilution of 1:400 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 15021-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15021-1-AP (CITED2 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IF Staining of SH-SY5Y using 15021-1-AP

  Immunofluorescent analysis of (10% Formaldehyde) fixed SH-SY5Y cells using 15021-1-AP (CITED2 antibody) at dilution of 1:50 and Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules SH-SY5Y, tissu cérébral de souris
• Résultats positifs en IF: cellules SH-SY5Y

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

15021-1-AP cible CITED2 dans les applications de WB, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CITED2 Protéine recombinante Ag7078
• Nom complet: Cbp/p300-interacting transactivator, with Glu/Asp-rich carboxy-terminal domain, 2
• Masse moléculaire calculée: 28 kDa
• Poids moléculaire observé: 36 kDa, 27 kDa
• Numéro d’acquisition GenBank: BC004377
• Symbole du gène: CITED2
• Identification du gène (NCBI): 10370
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CITED2, also named as P35srj, is a cAMP-responsive element-binding protein (CBP)/p300-transactivator, with an ED(Glu/Asp)-rich C-terminal domain that acts as an important modulator in the development of the heart [PMID:10552932]. There are two isoforms of CITED2 protein and the calculated molecular weight of them are 24kDa and 28 kDa. The 28 kDa isoform having modification site may migrate to 35 kDa and 24 kDa isoform having modification site may migrate to 27 kDa (PMID: 23082118). It is proposed to be a negative regulator of HIF-1α through its competitive binding to the CH1 domain of CBP/p300, which mediated signaling and to a decrease in HIF-1-responsive genes such as VEGF [PMID:9887100]. In addition, it is also a co-activator of the transcriptional activity of the TFAP2 isoform, which is an extensively expressed nuclear protein with functions in embryogenesis, inflammation, and stress responses[PMID:22735262].

Publications

Species	Application	Title
mouse	WB	Toxicol Sci; The role of miR-182-5p in hepatocarcinogenesis of trichloroethylene in mice.; Authors - Yan Jiang