Anticorps Polyclonal de lapin anti-CKAP4

CKAP4 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications

34

Avis (1)

N° de cat : 16686-1-AP

Synonymes

63 kDa membrane protein, CKAP4, CLIMP 63, ERGIC 63, p63

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Galerie de données de validation

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  WB analysis using 16686-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16686-1-AP (CKAP4 antibody) at dilution of 1:40000 incubated at room temperature for 1.5 hours.

• 

  WB analysis using 16686-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 16686-1-AP (CKAP4 antibody) at dilution of 1:60000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 16686-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 16686-1-AP (CKAP4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 16686-1-AP

  IP Result of anti-CKAP4 (IP:16686-1-AP, 4ug; Detection:16686-1-AP 1:1000) with HeLa cells lysate 2000ug.

• 

  IHC staining of human kidney using 16686-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16686-1-AP (CKAP4 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 16686-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 16686-1-AP (CKAP4 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver using 16686-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 16686-1-AP (CKAP4 Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 16686-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver slide using 16686-1-AP (CKAP4 Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 16686-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CKAP4 antibody (16686-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-Phalloidin (red).

• 

  IF Staining of HepG2 using 16686-1-AP

  Immunofluorescent analysis of HepG2 cells, using CKAP4 antibody 16686-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

• 

  IF Staining of HepG2 using 16686-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using CKAP4 antibody (16686-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules A431, cellules HeLa, cellules HepG2, cellules NIH/3T3, tissu rénal de rat
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IHC: tissu rénal humain, tissu hépatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 21 publications below
• IHC: See 2 publications below
• IF: See 12 publications below
• IP: See 4 publications below
• CoIP: See 1 publications below

Informations sur le produit

16686-1-AP cible CKAP4 dans les applications de WB, IP, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CKAP4 Protéine recombinante Ag10019
• Nom complet: cytoskeleton-associated protein 4
• Masse moléculaire calculée: 602 aa, 66 kDa
• Poids moléculaire observé: 63 kDa
• Numéro d’acquisition GenBank: BC082972
• Symbole du gène: CKAP4
• Identification du gène (NCBI): 10970
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CKAP4, also named as Climp-63, is a 63 kDa cytoskeleton-linking membrane protein. It is the partner of triadin. CKAP4 is responsible for this association of triads and microtubules.

Publications

Species	Application	Title
mouse	WB	Nature; Regulation of endoplasmic reticulum turnover by selective autophagy.; Authors - Aliaksandr Khaminets
rat	WB	Cell Metab; Palmitoylation couples insulin hypersecretion with β cell failure in diabetes; Authors - Guifang Dong
human	WB	Mol Cell; Quality Control of ER Membrane Proteins by the RNF185/Membralin Ubiquitin Ligase Complex; Authors - Michael L van de Weijer
human	WB	Mol Cell; Quality Control of ER Membrane Proteins by the RNF185/Membralin Ubiquitin Ligase Complex.; Authors - Michael L van de Weijer
mouse	IF	EMBO J; ER-to-lysosome-associated degradation of proteasome-resistant ATZ polymers occurs via receptor-mediated vesicular transport.; Authors - Ilaria Fregno
	IF	EMBO J; MiT/TFE factors control ER-phagy via transcriptional regulation of FAM134B.; Authors - Laura Cinque

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

WEI (Verified Customer) (06-28-2020)

Single strong band at correct size.

• Applications: Western Blot,
• Primary Antibody Dilution: 1:3000
• Cell Tissue Type: Mouse Heart

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