Anticorps Monoclonal anti-CKB/CKM

• Phare
• Validé par KD/KO

CKB/CKM Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG2a

Réactivité testée

Humain, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications(3)

CloneNo.

2B7C3

N° de cat : 66764-1-Ig

Synonymes

CKB, KCRB, 2B7C3, B CK, B-CK

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Galerie de données de validation

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  WB analysis of HEK-293 using 66764-1-Ig

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 66764-1-Ig (CKB/CKM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 66764-1-Ig

  HeLa cells were subjected to SDS PAGE followed by western blot with 66764-1-Ig (CKB/CKM antibody) at dilution of 1:10000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse brain using 66764-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66764-1-Ig (CKB/CKM antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 66764-1-Ig

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 66764-1-Ig (CKB/CKM antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules HeLa
• Résultats positifs en IHC: tissu cérébral de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:10000-1:50000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IHC: See 1 publications below

Informations sur le produit

66764-1-Ig cible CKB/CKM dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Mouse / IgG2a
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CKB/CKM Protéine recombinante Ag7933
• Nom complet: creatine kinase, brain
• Masse moléculaire calculée: 43 kDa
• Poids moléculaire observé: 43 kDa
• Numéro d’acquisition GenBank: BC001190
• Symbole du gène: CKB
• Identification du gène (NCBI): 1152
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine A
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CKBB, also named as B-CK and CKB, is a member of the ATP:guanido phosphotransferase protein family. It is a cytoplasmic enzyme involved in energy homeostasis. CKBB reversibly catalyzes the transfer of phosphate between ATP and various phosphogens such as creatine phosphate. It acts as a homodimer in brain as well as in other tissues, and as a heterodimer with a similar muscle isozyme in heart. CK isoenzymes play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. CK MB consists of a dimer of nonidentical chains. With MM being the major form in skeletal muscle and myocardium, MB existing in myocardium, and BB existing in many tissues, especially brain. This antibody can recognize both CKB and CKM due to the high homology.

Publications

Species	Application	Title
human	IHC	Theranostics; Single-cell analyses reveal evolution mimicry during the specification of breast cancer subtype; Authors - Zhi-Jie Gao
human	WB	Pharmacol Res; Ginkgolide B targets and inhibits creatine kinase B to regulate the CCT/TRiC-SK1 axis and exerts pro-angiogenic activity in middle cerebral artery occlusion mice.; Authors - Jiale Zhu ; KD Validated
human	WB	Transl Oncol; RNA-binding protein IGF2BP2 suppresses metastasis of clear cell renal cell carcinoma by enhancing CKB mRNA stability and expression; Authors - Junwu Ren

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