Anticorps Polyclonal de lapin anti-CKM/CKB

CKM/CKB Polyclonal Antibody for IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 15891-1-AP

Synonymes

CKM, CKM/CKB, CKMM, Creatine Kinase, Creatine Kinase MM, Creatine kinase M chain, Creatine kinase M type, creatine kinase, muscle, M CK

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Galerie de données de validation

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  WB analysis of mouse heart using 15891-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15891-1-AP (Creatine Kinase antibody at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse skeletal muscle using 15891-1-AP

  mouse skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 15891-1-AP (Creatine Kinase antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse brain using 15891-1-AP

  mouse brain tissue were subjected to SDS PAGE followed by western blot with 15891-1-AP (Creatine Kinase antibody at dilution of 1:8000 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 15891-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 15891-1-AP (CKM/CKB antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat heart using 15891-1-AP

  Immunohistochemical analysis of paraffin-embedded rat heart tissue slide using 15891-1-AP (CKM/CKB antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, tissu cérébral de souris, tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu cardiaque de souris, tissu cardiaque de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below

Informations sur le produit

15891-1-AP cible CKM/CKB dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CKM/CKB Protéine recombinante Ag8678
• Nom complet: creatine kinase, muscle
• Masse moléculaire calculée: 381 aa, 43 kDa
• Poids moléculaire observé: 43 kDa
• Numéro d’acquisition GenBank: BC007462
• Symbole du gène: CKM
• Identification du gène (NCBI): 1158
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Creatine Kinase, an enzyme important for energy metabolism in cells of high and fluctuating energy requirements, catalyses the reversible transfer of a phosphoryl goup from phosphocreatine to ADP. CK isoenzymes(muscle, brain, sarcomeric, Ubiquitous-type) play a central role in energy transduction in tissues with large, fluctuating energy demands, such as skeletal muscle, heart, brain and spermatozoa. Inactivation of creatine kinase by gliotoxin was accompanied by the formation of a 37-kDa form of the enzyme.This oxidized form of creatine kinase was rapidly reconverted to the 42-kDa species by the addition of reducing agents concomitant with restoration of activity.(PMID: 10827185). This antibody can recognize both CKB and CKM due to the high homology.

Publications

Species	Application	Title
rat	WB	Lipids Health Dis; Dietary fish oil supplement induces age-specific contractile and proteomic responses in muscles of male rats.; Authors - David W Russ
human	WB	Spinal Cord; The relationship between skin ultrasound images and muscle damage using skin blotting in wheelchair basketball athletes.; Authors - Nao Tamai
human	WB	Nanomedicine; Chemosensitization and mitigation of Adriamycin-induced cardiotoxicity using combinational polymeric micelles for co-delivery of quercetin/resveratrol and resveratrol/curcumin in ovarian cancer.; Authors - Adel Al Fatease