Anticorps Polyclonal de lapin anti-CKMT1A

• Phare
• Validé par KD/KO

CKMT1A Polyclonal Antibody for WB, IHC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, IP, ELISA

Conjugaison

Non conjugué

Publications(21)

Avis (1)

N° de cat : 15346-1-AP

Synonymes

Creatine kinase U-type, mitochondrial, CKMT1B, CKMT1, CKMT, CKMiMi

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Galerie de données de validation

• 

  WB analysis of A549 cells using 15346-1-AP

  A549 cells (shcontrol and shRNA of CKMT1A) were subjected to SDS PAGE followed by western blot with 15346-1-AP (CKMT1A antibody) at dilution of 1:30.

• 

  WB analysis of rat heart using 15346-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 15346-1-AP (CKMT1A Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 15346-1-AP

  WB result of CKMT1A antibody (15346-1-AP; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CKMT1A transfected HEK-293 cells.

• 

  WB analysis of human colon using 15346-1-AP

  human colon tissue were subjected to SDS PAGE followed by western blot with 15346-1-AP (CKMT1A antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human testis using 15346-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 15346-1-AP (CKMT1A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 15346-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15346-1-AP (CKMT1A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse testis using 15346-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 15346-1-AP (CKMT1A Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse heart using 15346-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 15346-1-AP (CKMT1A antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse heart using 15346-1-AP

  IP result of anti-CKMT1A (IP:15346-1-AP, 4ug; Detection:15346-1-AP 1:1000) with mouse heart tissue lysate 3200ug.

• 

  IHC staining of human colon cancer using 15346-1-AP

  Immunohistochemical analysis of paraffin-embedded human colon cancer tissue slide using 15346-1-AP (CKMT1A antibody) at dilution of 1:800 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 15346-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 15346-1-AP (CKMT1A antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de rat, cellules A549, cellules HEK-293, tissu cardiaque de souris, tissu de côlon humain, tissu testiculaire de souris, tissu testiculaire humain
• Résultats positifs en IP: tissu cardiaque de souris
• Résultats positifs en IHC: tissu de cancer du côlon humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:3000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:400-1:1600

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 3 publications below
• WB: See 14 publications below
• IHC: See 8 publications below
• IF: See 6 publications below
• FC: See 1 publications below

Informations sur le produit

15346-1-AP cible CKMT1A dans les applications de WB, IHC, IF, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CKMT1A Protéine recombinante Ag7583
• Nom complet: creatine kinase, mitochondrial 1A
• Masse moléculaire calculée: 47 kDa
• Poids moléculaire observé: 40-50 kDa
• Numéro d’acquisition GenBank: BC001926
• Symbole du gène: CKMT1A
• Identification du gène (NCBI): 548596
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CKMT1A(Creatine kinase U-type, mitochondrial) is also named CKMT and belongs to the ATP:guanido phosphotransferase family. CKMT1A is a nearly identical copy of the CKMT1B gene. Both genes encode an identical CKMT1 protein(PMID:17098888). It reversibly catalyzes the transfer of phosphate between ATP and various phosphates. It has 2 isoforms produced by alternative splicing with the molecular weight of 47 kDa and 50 kDa.

Publications

Species	Application	Title
human	WB	Cell Metab; Tyrosine Phosphorylation of Mitochondrial Creatine Kinase 1 Enhances a Druggable Tumor Energy Shuttle Pathway.; Authors - Kiran Kurmi ; KD Validated
human	WB,IHC,IF	Nat Commun; Functional antagonism between ΔNp63α and GCM1 regulates human trophoblast stemness and differentiation.; Authors - Liang-Jie Wang ; KD Validated
human	IHC, WB	Respir Res; Mitochondrial creatine kinase 1 in non-small cell lung cancer progression and hypoxia adaptation.; Authors - Ming Li
human	WB	Mol Oncol; Ryanodine receptor 2 promotes colorectal cancer metastasis by the ROS/BACH1 axis; Authors - Tianwei Chen
mouse	IHC	Int J Cancer; Increased serum mitochondrial creatine kinase activity as a risk for hepatocarcinogenesis in chronic hepatitis C patients.; Authors - Kenichiro Enooku
rat	IHC	Front Cell Neurosci; Investigations into photoreceptor energy metabolism during experimental retinal detachment; Authors - Glyn Chidlow

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Nusrat (Verified Customer) (08-09-2024)

The antibody yielded a strong, prominent band at about 42 kD and works superbly. It also works well at a 1:1000 dilution.

• Applications: Western Blot
• Primary Antibody Dilution: 1:500
• Cell Tissue Type: Porcine HCC cell line

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