Anticorps Polyclonal de lapin anti-CKMT2

• Phare
• Validé par KD/KO

CKMT2 Polyclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF, CoIP, ELISA

Conjugaison

Non conjugué

Publications(11)

Avis (1)

N° de cat : 13207-1-AP

Synonymes

Basic-type mitochondrial creatine kinase, CKMT 2, Creatine kinase S-type, mitochondrial, EC:2.7.3.2, Mib CK

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Galerie de données de validation

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  WB analysis using 13207-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 13207-1-AP (CKMT2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 13207-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 13207-1-AP (CKMT2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 13207-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 13207-1-AP (CKMT2 antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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  IHC staining of mouse heart using 13207-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13207-1-AP (CKMT2 antibody) at dilution of 1:1000 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 13207-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13207-1-AP (CKMT2 antibody) at dilution of 1:1000 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse heart using 13207-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13207-1-AP (CKMT2 antibody) at dilution of 1:500 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse heart using 13207-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse heart tissue slide using 13207-1-AP (CKMT2 antibody) at dilution of 1:500 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, tissu cardiaque humain, tissu de muscle squelettique de souris, tissu de muscle squelettique humain
• Résultats positifs en IHC: tissu cardiaque de souris, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:8000
• Immunohistochimie (IHC): IHC : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 8 publications below
• IHC: See 2 publications below
• IF: See 1 publications below
• CoIP: See 1 publications below

Informations sur le produit

13207-1-AP cible CKMT2 dans les applications de WB, IHC, IF, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CKMT2 Protéine recombinante Ag3971
• Nom complet: creatine kinase, mitochondrial 2 (sarcomeric)
• Masse moléculaire calculée: 419 aa, 48 kDa
• Poids moléculaire observé: 41-48 kDa
• Numéro d’acquisition GenBank: BC029140
• Symbole du gène: CKMT2
• Identification du gène (NCBI): 1160
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CKMT2 (Creatine kinase S-type, mitochondrial), also known as s-MtCK, is one of the creatine kinase (CK) isozymes that are tightly coupled to adenosine triphosphate (ATP) output via adenine nucleotide transport proteins or carriers, making it an important player in ATP synthesis and respiratory chain activity (PMID: 38172162). Enhancing Ckmt2 expression or activity can protect against myocardial injury caused by ischemia-reperfusion injury (PMID: 39457679).

Publications

Species	Application	Title
mouse	WB	Cell Metab; The phytochemical hyperforin triggers thermogenesis in adipose tissue via a Dlat-AMPK signaling axis to curb obesity.; Authors - Suzhen Chen
chicken	WB	Mol Cell Proteomics; Seminal Plasma Proteome as an Indicator of Sperm Dysfunction and Low Sperm Motility in Chickens; Authors - Yunlei Li
mouse	WB,IHC	Metabolism; Sustained mitochondrial biogenesis is essential to maintain caloric restriction-induced beige adipocytes.; Authors - Raja Gopal Reddy Mooli
human	WB	Front Cell Dev Biol; Hypoxia-immune-related microenvironment prognostic signature for osteosarcoma; Authors - Wenshuo Zhang
chicken	WB	Mol Cell Proteomics; Seminal Plasma Proteome as an Indicator of Sperm Dysfunction and Low Sperm Motility in Chickens.; Authors - Yunlei Li
mouse	WB	J Agric Food Chem; Artepillin C, a key component of Brazilian propolis, induces thermogenesis in inguinal white adipose tissue of mice through a creatine metabolism-related thermogenic pathway.; Authors - Sho Nishikawa

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Neeraj (Verified Customer) (08-19-2025)

This antibody performed very well in validating my Western blot analysis following transfection in cell lines. It demonstrated high specificity, especially when compared to a similar antibody from another supplier, which showed non-specific binding. I also used it for immunofluorescence experiments, and the results were satisfactory, with clear and consistent staining

• Applications: Western Blot, Immunohistochemistry, Immunofluorescence
• Primary Antibody Dilution: 1:1000 for western blot; 1:200 for Immunostaining
• Cell Tissue Type: Retinal sections, organoids, HEK cell lines

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