Anticorps Polyclonal de lapin anti-CLCNKA

CLCNKA Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

N° de cat : 14402-1-AP

Synonymes

chloride channel Ka, ClC K1, CLCK1, CLCNKA, hClC Ka

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Galerie de données de validation

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  WB analysis using 14402-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14402-1-AP (CLCNKA antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of human kidney using 14402-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 14402-1-AP (CLCNKA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of mouse pancreas using 14402-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 14402-1-AP (CLCNKA antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of HEK-293 using 14402-1-AP

  IP Result of anti-CLCNKA (IP:14402-1-AP, 5ug; Detection:14402-1-AP 1:500) with HEK-293 cells lysate 880ug.

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  IHC staining of human kidney using 14402-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14402-1-AP (CLCNKA antibody) at dilution of 1:800 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human kidney using 14402-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 14402-1-AP (CLCNKA antibody) at dilution of 1:800 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human ovary using 14402-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14402-1-AP (CLCNKA antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human ovary using 14402-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary using 14402-1-AP (CLCNKA antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 14402-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14402-1-AP (CLCNKA antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human placenta using 14402-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta using 14402-1-AP (CLCNKA antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human testis using 14402-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14402-1-AP (CLCNKA antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human testis using 14402-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 14402-1-AP (CLCNKA antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human skin using 14402-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14402-1-AP (CLCNKA antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human skin using 14402-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 14402-1-AP (CLCNKA antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human spleen using 14402-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14402-1-AP (CLCNKA antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human spleen using 14402-1-AP

  Immunohistochemical analysis of paraffin-embedded human spleen using 14402-1-AP (CLCNKA antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human lung using 14402-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14402-1-AP (CLCNKA antibody) at dilution of 1:100 (under 10x lens).

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  IHC staining of human lung using 14402-1-AP

  Immunohistochemical analysis of paraffin-embedded human lung using 14402-1-AP (CLCNKA antibody) at dilution of 1:100 (under 40x lens).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, tissu pancréatique de souris, tissu rénal de souris, tissu rénal humain
• Résultats positifs en IP: cellules HEK-293
• Résultats positifs en IHC: tissu rénal humain, tissu cutané humain, tissu ovarien humain, tissu placentaire humain, tissu pulmonaire humain, tissu splénique humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:400-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

14402-1-AP cible CLCNKA dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CLCNKA Protéine recombinante Ag5647
• Nom complet: chloride channel Ka
• Masse moléculaire calculée: 75 kDa
• Poids moléculaire observé: 70 kDa
• Numéro d’acquisition GenBank: BC048282
• Symbole du gène: CLCNKA
• Identification du gène (NCBI): 1187
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

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