Anticorps Polyclonal de lapin anti-Claudin 11

Claudin 11 Polyclonal Antibody for FC, IF, IHC, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris et plus (1)

Applications

WB, IHC, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

4

N° de cat : 12152-1-AP

Synonymes

claudin 11, CLDN11, OSP, OTM

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Galerie de données de validation

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  WB analysis of human testis using 12152-1-AP

  human testis tissue were subjected to SDS PAGE followed by western blot with 12152-1-AP (Claudin 11 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IHC staining of human testis using 12152-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12152-1-AP (Claudin 11 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 12152-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 12152-1-AP (Claudin 11 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of mouse brain using 12152-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12152-1-AP (Claudin 11 antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse brain using 12152-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse brain tissue slide using 12152-1-AP (Claudin 11 antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of HepG2 using 12152-1-AP

  Immunofluorescent analysis of HepG2 cells, using CLDN11 antibody 12152-1-AP at 1:100 dilution and PE-labeled goat anti-rabbit IgG(red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  FC experiment of HepG2 using 12152-1-AP

  1X10^6 HepG2 cells were stained with 0.2ug Claudin 11 antibody (12152-1-AP, red) and control antibody (blue). Fixed with 90% MeOH blocked with 3% BSA (30 min). Alexa Fluor 488-conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) with dilution 1:1000.

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Applications testées

• Résultats positifs en WB: tissu testiculaire humain
• Résultats positifs en IHC: tissu testiculaire humain, tissu cérébral de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HepG2
• Résultats positifs en cytométrie: cellules HepG2

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF): IF : 1:20-1:200
• Flow Cytometry (FC): FC : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 3 publications below
• IF: See 1 publications below

Informations sur le produit

12152-1-AP cible Claudin 11 dans les applications de WB, IHC, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: rat, Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Claudin 11 Protéine recombinante Ag2764
• Nom complet: claudin 11
• Masse moléculaire calculée: 207 aa, 22 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: BC013577
• Symbole du gène: CLDN11
• Identification du gène (NCBI): 5010
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 23 claudins have been identified. They are small (20-27 kilodalton (kDa)) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm.

Publications

Species	Application	Title
human	IF	Mol Cell Endocrinol; Cardiotonic steroid ouabain stimulates expression of blood-testis barrier proteins claudin-1 and -11 and formation of tight junctions in Sertoli cells.; Authors - Raimund Dietze
rat	WB	Environ Sci Pollut Res Int; Polystyrene microplastics induce blood-testis barrier disruption regulated by the MAPK-Nrf2 signaling pathway in rats.; Authors - Shengda Li
human	WB	J Physiol Biochem; α2,6-Sialylation promotes hepatocellular carcinoma cells migration and invasion via enhancement of nSmase2-mediated exosomal miRNA sorting; Authors - Liping Wang
human	WB	Sci Rep; Anti-breast Cancer Activity of SPG-56 from Sweet Potato in MCF-7 Bearing Mice in Situ through Promoting Apoptosis and Inhibiting Metastasis.; Authors - Zhaoxing Li

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