Anticorps Polyclonal de lapin anti-Claudin 3

Claudin 3 Polyclonal Antibody for IHC, IP,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

31

Avis (2)

N° de cat : 16456-1-AP

Synonymes

C7orf1, claudin 3, Claudin3, CLDN3, CPE R 2, CPE R2, CPE receptor 2, CPETR2, HRVP1, RVP1

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Galerie de données de validation

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  IP experiment of mouse large intestine using 16456-1-AP

  IP Result of anti-Claudin 3 (IP:16456-1-AP, 4ug; Detection:16456-1-AP 1:300) with mouse large intestine tissue lysate 3200ug.

• 

  IHC staining of human breast cancer using 16456-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16456-1-AP (Claudin 3 Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 16456-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16456-1-AP (Claudin 3 Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of mouse colon using 16456-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 16456-1-AP (Claudin 3 Antibody) at dilution of 1:200 (under 10x lens).

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  IHC staining of mouse colon using 16456-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse colon tissue slide using 16456-1-AP (Claudin 3 Antibody) at dilution of 1:200 (under 40x lens).

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Applications testées

• Résultats positifs en IP: tissu de gros intestin de souris
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de côlon de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• IHC: See 8 publications below
• IF: See 9 publications below

Informations sur le produit

16456-1-AP cible Claudin 3 dans les applications de IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Claudin 3 Protéine recombinante Ag9411
• Nom complet: claudin 3
• Masse moléculaire calculée: 220 aa, 23 kDa
• Poids moléculaire observé: 20-24 kDa
• Numéro d’acquisition GenBank: BC016056
• Symbole du gène: CLDN3
• Identification du gène (NCBI): 1365
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Claudins are a family of proteins that are the most important components of the tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. 23 claudins have been identified. They are small (20-27 kilodalton (kDa)) proteins with very similar structure. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm.

Publications

Species	Application	Title
human	IHC	Aging Cell; Advanced maternal age causes premature placental senescence and malformation via dysregulated α-Klotho expression in trophoblasts.; Authors - Zhi Chen
mouse	IF	EMBO Mol Med; Deficiency in intestinal epithelial O-GlcNAcylation predisposes to gut inflammation.; Authors - Ming Zhao
mouse,rat	IHC	J Hazard Mater; Dual effects of JNK activation in blood-milk barrier damage induced by zinc oxide nanoparticles.; Authors - Junrong Wu
mouse	IHC	Environ Int; Translocation of transition metal oxide nanoparticles to breast milk and offspring: The necessity of bridging mother-offspring-integration toxicological assessments.; Authors - Jie Cai
mouse		Biochem Pharmacol; Inhibition of S100A9 alleviates neurogenic pulmonary edema after subarachnoid hemorrhage; Authors - Guijun Wang
mouse	IHC	NanoImpact; Altered protein S-glutathionylation depicts redox imbalance triggered by transition metal oxide nanoparticles in a breastfeeding system.; Authors - Jie Cai

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive forproviding their feedback.

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FH

Priya (Verified Customer) (07-31-2023)

Used for Caco2 cells and mice tissue

• Applications: Western Blot
• Primary Antibody Dilution: 1 to 1000
• Cell Tissue Type: Caco2, Mice colon

FH

Priya (Verified Customer) (06-21-2023)

Used this antibody for Caco2 cells andmice tissue

• Applications: Western Blot
• Primary Antibody Dilution: 1 to 1000
• Cell Tissue Type: Caco2, Mice colon

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