Anticorps Polyclonal de lapin anti-Claudin 4-specific

• Phare
• Validé par KD/KO

Claudin 4-specific Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, RIP, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

37

N° de cat : 16195-1-AP

Synonymes

claudin 4, Claudin 4-specific, CLDN4, CPE R, CPE receptor, CPER, CPETR, CPETR1, hCPE R, WBSCR8

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Galerie de données de validation

• 

  WB analysis of MDA-MB-453s using 16195-1-AP

  MDA-MB-453s cells were subjected to SDS PAGE followed by western blot with 16195-1-AP (Claudin 4-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse small intestine using 16195-1-AP

  mouse small intestine tissue were subjected to SDS PAGE followed by western blot with 16195-1-AP (CLDN4-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 16195-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 16195-1-AP (CLDN4-specific antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of human placenta using 16195-1-AP

  IP Result of anti-Claudin 4-specific (IP:16195-1-AP, 4ug; Detection:16195-1-AP 1:300) with human placenta tissue lysate 4400ug.

• 

  IHC staining of human breast cancer using 16195-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16195-1-AP (Claudin 4-specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 16195-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16195-1-AP (Claudin 4-specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 16195-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16195-1-AP (Claudin 4-specific antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human ovary tumor using 16195-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16195-1-AP (Claudin 4-specific antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 16195-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16195-1-AP (Claudin 4-specific Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 16195-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 16195-1-AP (Claudin 4-specific Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human ovary tumor using 16195-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16195-1-AP (Claudin 4-specific Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human ovary tumor using 16195-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary tumor tissue slide using 16195-1-AP (Claudin 4-specific Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of human breast cancer using 16195-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using Claudin 4-specific antibody (16195-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human breast cancer using 16195-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human breast cancer tissue using Claudin 4-specific antibody (16195-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: cellules MDA-MB-453s, tissu d'intestin grêle de souris, tissu placentaire humain
• Résultats positifs en IP: tissu placentaire humain
• Résultats positifs en IHC: tissu de cancer du sein humain, tissu de tumeur ovarienne humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: tissu de cancer du sein humain,

Dilution recommandée

• Western Blot (WB): WB : 1:200-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 22 publications below
• IHC: See 12 publications below
• IF: See 7 publications below
• RIP: See 1 publications below

Informations sur le produit

16195-1-AP cible Claudin 4-specific dans les applications de WB, RIP, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, porc, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Peptide
• Nom complet: claudin 4
• Masse moléculaire calculée: 22 kDa
• Poids moléculaire observé: 22 kDa
• Numéro d’acquisition GenBank: NM_001305
• Symbole du gène: CLDN4
• Identification du gène (NCBI): 1364
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Claudins are a family of proteins that are the most important components of tight junctions, where they establish the paracellular barrier that controls the flow of molecules in the intercellular space between the cells of an epithelium. They are small (20-27 kDa) proteins with very similar structures. They have four transmembrane domains, with the N-terminus and the C-terminus in the cytoplasm. This antibody is specifically against claudin 4.

Publications

Species	Application	Title
human,mouse	IHC,IF	Cell Metab; Gut microbiota modulate distal symmetric polyneuropathy in patients with diabetes; Authors - Junpeng Yang
mouse,rat	WB,IHC	J Hazard Mater; Dual effects of JNK activation in blood-milk barrier damage induced by zinc oxide nanoparticles.; Authors - Junrong Wu
human	WB	Free Radic Biol Med; Prenylated xanthones from mangosteen (Garcinia mangostana) activate the AhR and Nrf2 pathways and protect intestinal barrier integrity in HT-29 cells.; Authors - Restituto Tocmo
human	WB, RIP	Cancers (Basel); Targeting PVT1 Exon 9 Re-Expresses Claudin 4 Protein and Inhibits Migration by Claudin-Low Triple Negative Breast Cancer Cells.; Authors - Fayola Levine
rat	WB	EBioMedicine; Akkermansia muciniphila prevents cold-related atrial fibrillation in rats by modulation of TMAO induced cardiac pyroptosis.; Authors - Yingchun Luo
human	WB	Cancer Sci; Targeting claudin-4 enhances chemosensitivity in breast cancer.; Authors - Yi Luo

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