Anticorps Polyclonal de lapin anti-CLEC12A

CLEC12A Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, ELISA

Conjugaison

Non conjugué

N° de cat : 28627-1-AP

Synonymes

C type lectin like molecule 1, CLEC12A, CLL 1, CLL1, DCAL 2, DCAL2, MICL

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Galerie de données de validation

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  WB analysis of U-937 using 28627-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 28627-1-AP (CLEC12A antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis using 28627-1-AP

  Human peripheral blood leukocytes were subjected to SDS PAGE followed by western blot with 28627-1-AP (CLEC12A antibody) at dilution of 1:800 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: cellules U-937, leucocyte de sang périphérique humain

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

28627-1-AP cible CLEC12A dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CLEC12A Protéine recombinante Ag27864
• Nom complet: C-type lectin domain family 12, member A
• Masse moléculaire calculée: 31 kDa
• Poids moléculaire observé: 55-75 kDa
• Numéro d’acquisition GenBank: BC063424
• Symbole du gène: CLEC12A
• Identification du gène (NCBI): 160364
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CLEC12A, also known as CD371, MICL, DCAL-2 or CLL-1, is a type II transmembrane glycoprotein and a member of the C-type lectin superfamily (PMID: 30401586). This protein, which contains a single C-type lectin-like domain and a cytoplasmic immunoreceptor tyrosine-based inhibitory motif (ITIM), is related to LOX-1 and Dectin-1 and is variably spliced and highly N-glycosylated (PMID: 14739280). Its ITIM sequence recruits the tyrosine phosphatases SHP-1 and SHP-2 to negatively regulate Syk signaling. In humans, CLEC12A is mainly expressed on myeloid cells, being found on monocytes, granulocytes, macrophages and DC subsets, and at lower levels on CD4+ T cells (PMID: 32477350). The observed molecular weight of CLEC12A is between 40 and 75 kDa, which is much higher than the predicted molecular weight due to glycosylation (PMID: 14739280).