Anticorps Monoclonal anti-CLIC4

• Phare
• Validé par KD/KO

CLIC4 Monoclonal Antibody for WB, IHC, IF/ICC, FC (Intra), ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat

Applications

WB, IHC, IF/ICC, FC (Intra), ELISA

Conjugaison

Non conjugué

Publications(1)

CloneNo.

1B7F9

N° de cat : 66343-1-Ig

Synonymes

MTCLIC, huH1, H1, FLJ38640, CLIC4L

---

Galerie de données de validation

• 

  WB analysis of HeLa using 66343-1-Ig

  WB result of CLIC4 antibody (66343-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CLIC4 transfected HeLa cells.

• 

  WB analysis using 66343-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66343-1-Ig (CLIC4 antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of pig heart using 66343-1-Ig

  pig heart tissue were subjected to SDS PAGE followed by western blot with 66343-1-Ig (CLIC4 Antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human heart using 66343-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66343-1-Ig (CLIC4 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human heart using 66343-1-Ig

  Immunohistochemical analysis of paraffin-embedded human heart tissue slide using 66343-1-Ig (CLIC4 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IHC staining of human kidney using 66343-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66343-1-Ig (CLIC4 Antibody) at dilution of 1:200 (under 10x lens).

• 

  IHC staining of human kidney using 66343-1-Ig

  Immunohistochemical analysis of paraffin-embedded human kidney tissue slide using 66343-1-Ig (CLIC4 Antibody) at dilution of 1:200 (under 40x lens).

• 

  IF Staining of HeLa using 66343-1-Ig

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CLIC4 antibody (66343-1-Ig, Clone: 1B7F9 ) at dilution of 1:2000 and CoraLite®488-Conjugated Goat Anti-Mouse IgG(H+L) (SA00013-1).

• 

  FC experiment of BT-549 using 66343-1-Ig

  1x10^6 BT-549 cells were intracellularly stained with 0.2 μg CLIC4 Monoclonal antibody (66343-1-Ig, Clone: 1B7F9) and CoraLite® Plus 647-Goat Anti-Mouse Recombinant Secondary Antibody (H+L)(Cat.NO.RGAM005). Mouse IgG1 isotype control (66360-1-Ig, Clone: 1F8D3, blue) was parallel stained as control. Cells were fixed with 4% PFA.

---

Applications testées

• Résultats positifs en WB: cellules HuH-7, cellules HeLa, cellules U2OS, tissu cardiaque de porc, tissu cérébral de rat, tissu cérébral de souris, tissu rénal de lapin, tissu rénal de porc, tissu rénal de rat, tissu rénal de souris
• Résultats positifs en IHC: tissu cardiaque humain, tissu rénal humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,
• Résultats positifs en FC (Intra): BT-549 cells,

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:1000-1:4000
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.20 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below
• IF: See 1 publications below

Informations sur le produit

66343-1-Ig cible CLIC4 dans les applications de WB, IHC, IF/ICC, FC (Intra), ELISA et montre une réactivité avec des échantillons Humain, porc, rat

• Réactivité: Humain, porc, rat
• Réactivité citée: rat
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CLIC4 Protéine recombinante Ag2942
• Nom complet: chloride intracellular channel 4
• Masse moléculaire calculée: 253 aa, 29 kDa
• Poids moléculaire observé: 29 kDa
• Numéro d’acquisition GenBank: BC012444
• Symbole du gène: CLIC4
• Identification du gène (NCBI): 25932
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Chloride channels are a diverse group of proteins that regulate fundamental cellular processes including stabilization of cell membrane potential, transepithelial transport, maintenance of intracellular pH, and regulation of cell volume. CLIC4 is a p53- and tumor necrosis factor alpha-regulated cytoplasmic and mitochondrial protein that belongs to the CLIC family of intracellular chloride channels. CLICs have actions distinct from traditional cell membrane chloride channels, including formation of ion channels in intracellular organelles and roles in membrane trafficking, apoptosis, and cell differentiation.

Publications

Species	Application	Title
rat	WB,IF	Oxid Med Cell Longev; Attenuation of ROS/Chloride Efflux-Mediated NLRP3 Inflammasome Activation Contributes to Alleviation of Diabetic Cardiomyopathy in Rats after Sleeve Gastrectomy.; Authors - Songhan Li

{{ptg:RelatedPrimaryAntibodies}}