Anticorps Polyclonal de lapin anti-CLIP1

CLIP1 Polyclonal Antibody for WB, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IF/ICC, IP, ELISA

Conjugaison

Non conjugué

Publications(5)

N° de cat : 23839-1-AP

Synonymes

CAP-Gly domain-containing linker protein 1, CLIP-170, CYLN1, Cytoplasmic linker protein 170 alpha-2, Reed-Sternberg intermediate filament-associated protein

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Galerie de données de validation

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  WB analysis using 23839-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 23839-1-AP (CLIP1 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HEK-293 using 23839-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23839-1-AP (CLIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 23839-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 23839-1-AP (CLIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of U-937 using 23839-1-AP

  U-937 cells were subjected to SDS PAGE followed by western blot with 23839-1-AP (CLIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 23839-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23839-1-AP (CLIP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 23839-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 23839-1-AP (CLIP1 antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HEK-293 using 23839-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 23839-1-AP (CLIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of rat kidney using 23839-1-AP

  rat kidney tissue were subjected to SDS PAGE followed by western blot with 23839-1-AP (CLIP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse kidney using 23839-1-AP

  mouse kidney tissue were subjected to SDS PAGE followed by western blot with 23839-1-AP (CLIP1 antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 23839-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 23839-1-AP (CLIP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  IP experiment of HeLa using 23839-1-AP

  IP result of anti-CLIP1 (IP:23839-1-AP, 4ug; Detection:23839-1-AP 1:2000) with HeLa cells lysate 2400ug.

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  IF Staining of HeLa using 23839-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using CLIP1 antibody (23839-1-AP) at dilution of 1:200 and Multi-rAb CoraLite ® Plus 488-Goat Anti-Rabbit Recombinant Secondary Antibody (H+L) (RGAR002), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HEK-293, cellules MCF-7, cellules U-937, tissu rénal de rat, tissu rénal de souris
• Résultats positifs en IP: cellules HeLa
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 4 publications below
• IF: See 1 publications below
• ELISA: See 1 publications below

Informations sur le produit

23839-1-AP cible CLIP1 dans les applications de WB, IF/ICC, IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CLIP1 Protéine recombinante Ag20811
• Nom complet: CAP-GLY domain containing linker protein 1
• Masse moléculaire calculée: 1438 aa, 162 kDa
• Poids moléculaire observé: 160-170 kDa, 135 kDa
• Numéro d’acquisition GenBank: BC126305
• Symbole du gène: CLIP1
• Identification du gène (NCBI): 6249
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purifié par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CLIP1, also known as RSN, CYLN1, is a member of the microtubule (MT) plus-end tracking protein family. CLIP1 associates with the ends of growing MTs. These proteins regulate MT dynamic behavior and are important for MT-mediated transport over the length of axons and dendrites. It has an important role in spermatogenesis as well as in neuronal development(PMID: 24569606).

Publications

Species	Application	Title
mouse	WB	Front Cell Dev Biol; TMPRSS12 Functions in Meiosis and Spermiogenesis and Is Required for Male Fertility in Mice.; Authors - Jingjing Zhang
human	WB	Cell Death Dis; Tension of plus-end tracking protein Clip170 confers directionality and aggressiveness during breast cancer migration; Authors - Yunfeng Hu
human	IF	Science; The mechanism of acentrosomal spindle assembly in human oocytes; Authors - Tianyu Wu
human	WB	Ecotoxicol Environ Saf; Exosomal circCLIP1 regulates PM2.5-induced airway obstruction via targeting SEPT10 in vitro; Authors - Huanhuan Zhu
rat,human	WB,ELISA	Exp Mol Med; Hypothermic oxygenated perfusion inhibits CLIP1-mediated TIRAP ubiquitination via TFPI2 to reduce ischemia‒reperfusion injury of the fatty liver; Authors - Pengpeng Yue

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