Anticorps Polyclonal de lapin anti-CLP1

CLP1 Polyclonal Antibody for WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, ELISA

Conjugaison

Non conjugué

Publications(2)

Avis (1)

N° de cat : 14746-1-AP

Synonymes

CLP1, hClp1, HEAB, Polynucleotide kinase Clp1

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Galerie de données de validation

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  WB analysis of mouse testis using 14746-1-AP

  mouse testis tissue were subjected to SDS PAGE followed by western blot with 14746-1-AP (CLP1 Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis using 14746-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14746-1-AP (CLP1 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 14746-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 14746-1-AP (CLP1 Antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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Applications testées

• Résultats positifs en WB: tissu testiculaire de souris, cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 1 publications below

Informations sur le produit

14746-1-AP cible CLP1 dans les applications de WB, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CLP1 Protéine recombinante Ag6479
• Nom complet: CLP1, cleavage and polyadenylation factor I subunit, homolog (S. cerevisiae)
• Masse moléculaire calculée: 48 kDa
• Poids moléculaire observé: 41 kDa, 48 kDa
• Numéro d’acquisition GenBank: BC000446
• Symbole du gène: CLP1
• Identification du gène (NCBI): 10978
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CLP1, also named as HEAB, is a 425 amino acid protein, which belongs to the Clp1 family. CLP1 as a polynucleotide kinase, that can phosphorylate the 5'-hydroxyl groups of double-stranded RNA (dsRNA), single-stranded RNA (ssRNA), double-stranded DNA (dsDNA) and double-stranded DNA:RNA hybrids. Human CLP1 is a component of the mRNA 3'end cleavage and polyadenylation machinery4,5 and studies in yeast have postulated a function for CLP1 in coupling mRNA 3' end processing with RNA Pol II transcriptional termination. CLP1 exists 2 isoforms with MV 41 kDa and 48 kDa.

Publications

Species	Application	Title
human	WB	Mol Cell; m6Am sequesters PCF11 to suppress premature termination and drive neuroblastoma differentiation; Authors - Huihui An
		Nucleic Acids Res; Multiple P-TEFbs cooperatively regulate the release of promoter-proximally paused RNA polymerase II.; Authors - Xiaodong Lu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Fabienne (Verified Customer) (09-12-2025)

Consistently detects the target protein in WB. Tested on HeLa total cell extracts

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa

CLP1 Antibody Western Blot validation (1:1000 dilution) in HeLa (Cat no:14746-1-AP)

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