Anticorps Polyclonal de lapin anti-CLPP

• Phare
• Validé par KD/KO

CLPP Polyclonal Antibody for WB, IHC, IF/ICC, IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (3)

Applications

WB, IHC, IF/ICC, IP, CoIP, ELISA

Conjugaison

Non conjugué

Publications(54)

Avis (2)

N° de cat : 15698-1-AP

Synonymes

Endopeptidase Clp, EC:3.4.21.92, ATP-dependent Clp protease proteolytic subunit, mitochondrial

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Galerie de données de validation

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  WB analysis using 15698-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 15698-1-AP (CLPP antibody) at dilution of 1:4000 incubated at room temperature for 1.5 hours.

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  WB analysis of rat heart using 15698-1-AP

  rat heart tissue were subjected to SDS PAGE followed by western blot with 15698-1-AP (CLPP Antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

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  WB analysis of K-562 using 15698-1-AP

  K-562 cells were subjected to SDS PAGE followed by western blot with 15698-1-AP (CLPP Antibody) at dilution of 1:1500 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse skeletal muscle using 15698-1-AP

  IP result of anti-CLPP (IP:15698-1-AP, 3ug; Detection:15698-1-AP 1:500) with mouse skeletal muscle tissue lysate 4000ug.

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  IHC staining of human liver cancer using 15698-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15698-1-AP (CLPP Antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human liver cancer using 15698-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15698-1-AP (CLPP Antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15698-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15698-1-AP (CLPP antibody) at dilution of 1:200 (under 10x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 15698-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 15698-1-AP (CLPP antibody) at dilution of 1:200 (under 40x lens. Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human kidney using 15698-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15698-1-AP (CLPP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human kidney using 15698-1-AP

  Immunohistochemical analysis of paraffin-embedded human kidney using 15698-1-AP (CLPP antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human testis using 15698-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15698-1-AP (CLPP antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human testis using 15698-1-AP

  Immunohistochemical analysis of paraffin-embedded human testis using 15698-1-AP (CLPP antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human liver using 15698-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15698-1-AP (CLPP antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 15698-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15698-1-AP (CLPP antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HeLa using 15698-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using CLPP antibody (15698-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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  IF Staining of HeLa using 15698-1-AP

  Immunofluorescent analysis of (-20°C Ethanol) fixed HeLa cells using 15698-1-AP (CLPP antibody) at dilution of 1:50 and CoraLite488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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Applications testées

• Résultats positifs en WB: tissu placentaire humain, cellules K-562, tissu cardiaque de rat, tissu cardiaque de souris
• Résultats positifs en IP: tissu de muscle squelettique de souris
• Résultats positifs en IHC: tissu de cancer du foie humain, tissu hépatique humain, tissu rénal humain, tissu testiculaire humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:8000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 4 publications below
• WB: See 50 publications below
• IHC: See 3 publications below
• IF: See 3 publications below
• CoIP: See 1 publications below

Informations sur le produit

15698-1-AP cible CLPP dans les applications de WB, IHC, IF/ICC, IP, CoIP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Chèvre, Humain, poisson-zèbre, poulet, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CLPP Protéine recombinante Ag8325
• Nom complet: ClpP caseinolytic peptidase, ATP-dependent, proteolytic subunit homolog (E. coli)
• Masse moléculaire calculée: 30 kDa
• Poids moléculaire observé: 26 kDa, 30 kDa
• Numéro d’acquisition GenBank: BC002956
• Symbole du gène: CLPP
• Identification du gène (NCBI): 8192
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CLPP is the putative ATP-dependent Clp protease proteolytic subunit and also named as endopeptidase Clp. It belongs to the peptidase S14 family. Clp cleaves peptides in various proteins in a process that requires ATP hydrolysis. It may be responsible for a fairly general and central housekeeping function rather than for the degradation of specific substrates. This protein has a transit peptide of 56 amino acid.

Publications

Species	Application	Title
	WB	Cell Metab; Fibroblast Growth Factor 21 Drives Dynamics of Local and Systemic Stress Responses in Mitochondrial Myopathy with mtDNA Deletions.; Authors - Saara Forsström
mouse	WB	Cell Metab; mTORC1 Regulates Mitochondrial Integrated Stress Response and Mitochondrial Myopathy Progression.; Authors - Nahid A Khan
human	WB	Mol Cell; The mitochondrial DNAJC co-chaperone TCAIM reduces α-ketoglutarate dehydrogenase protein levels to regulate metabolism; Authors - Wang Jiahui ; KD Validated
mouse	WB	Cell Metab; Proteolytic cleavage of opa1 stimulates mitochondrial inner membrane fusion and couples fusion to oxidative phosphorylation.; Authors - Prashant Mishra
mouse	WB	Sci Adv; Mitochondrial proteostasis stress in muscle drives a long-range protective response to alleviate dietary obesity independently of ATF4.; Authors - Qiqi Guo
mouse	WB	Nat Commun; Disuse-associated loss of the protease LONP1 in muscle impairs mitochondrial function and causes reduced skeletal muscle mass and strength.; Authors - Zhisheng Xu

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Anh (Verified Customer) (07-15-2021)

very good, very clear bands

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: heart cells

FH

Mariusz (Verified Customer) (02-06-2019)

Excellent antibody bot for Western blot (1:2000) dilution and immunofluorescence (1:200). Single band on Western, and clean signal reflecting mitochondrial matrix in IF.

• Applications: Western Blot, Immunofluorescence,
• Primary Antibody Dilution: 1:2000 (Western), 1:200 (IF)
• Cell Tissue Type: HeLa and HCt116

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