Anticorps Polyclonal de lapin anti-Clusterin

Clusterin Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain et plus (1)

Applications

WB, IHC, IF/ICC, IF-P, CoIP, ELISA

Conjugaison

Non conjugué

Publications(30)

Avis (1)

N° de cat : 12289-1-AP

Synonymes

AAG4, Aging-associated gene 4 protein, Apo J, APOJ, Apo-J

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Galerie de données de validation

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  WB analysis of human plasma using 12289-1-AP

  human plasma were subjected to SDS PAGE followed by western blot with 12289-1-AP (Clusterin antibody) at dilution of 1:6000 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human liver cancer using 12289-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12289-1-AP (Clusterin antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human liver cancer using 12289-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 12289-1-AP (Clusterin antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 12289-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12289-1-AP (Clusterin antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human tonsillitis using 12289-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 12289-1-AP (Clusterin antibody) at dilution of 1:600 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human breast cancer using 12289-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12289-1-AP (Clusterin antibody at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human breast cancer using 12289-1-AP

  Immunohistochemical analysis of paraffin-embedded human breast cancer tissue slide using 12289-1-AP (Clusterin antibody at dilution of 1:50 (under 40x lens).

• 

  IF Staining of human liver using 12289-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human liver tissue using Clusterin antibody (12289-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of human liver using 12289-1-AP

  Immunofluorescent analysis of (4% PFA) fixed human liver tissue using Clusterin antibody (12289-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

• 

  IF Staining of HeLa using 12289-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HeLa cells using Clusterin antibody (12289-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: plasma humain,
• Résultats positifs en IHC: tissu d'amygdalite humain, tissu de cancer du foie humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu hépatique humain, cellules HeLa
• Résultats positifs en IF/ICC: cellules HeLa,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:12000
• Immunohistochimie (IHC): IHC : 1:300-1:1200
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 17 publications below
• IHC: See 6 publications below
• IF: See 6 publications below
• CoIP: See 1 publications below

Informations sur le produit

12289-1-AP cible Clusterin dans les applications de WB, IHC, IF/ICC, IF-P, CoIP, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Réactivité citée: Humain, Lapin
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Clusterin Protéine recombinante Ag2889
• Nom complet: clusterin
• Masse moléculaire calculée: 449 aa, 53 kDa
• Poids moléculaire observé: 37 kDa
• Numéro d’acquisition GenBank: BC010514
• Symbole du gène: CLU
• Identification du gène (NCBI): 1191
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Clusterin (Apolipoprotein J) is a 75-80 kDa heterodimeric glycoprotein produced by a wide array of tissues and found in most biologic fluids (PMID: 7648419; 11906815). It is encoded by one single gene and cleaved posttranslationally into α (34-36 kDa) and β (36-39 kDa) subunits prior to secretion from the cell. Many functions have been described for clusterin, including regulating apoptosis, transporting lipids, controlling cell interactions, regulating complement (PMID: 10694874).

Publications

Species	Application	Title
		J Clin Invest; Chromosomal 3q amplicon encodes essential regulators of secretory vesicles that drive secretory addiction in cancer; Authors - Xiaochao Tan
human	WB	Proc Natl Acad Sci U S A; Addiction to Golgi-resident PI4P synthesis in chromosome 1q21.3-amplified lung adenocarcinoma cells.; Authors - Lei Shi
		Sci Signal; Platelets contribute to amyloid-β aggregation in cerebral vessels through integrin αIIbβ3-induced outside-in signaling and clusterin release.; Authors - Lili Donner
	WB	Front Cell Dev Biol; Structural and Functional Characterization of Fibronectin in Extracellular Vesicles From Hepatocytes.; Authors - Xinlei Li
human	WB	Ecotoxicol Environ Saf; Identification and functional analysis of senescence-associated secretory phenotype of premature senescent hepatocytes induced by hexavalent chromium.; Authors - Yu Ma
	IHC	Food Chem Toxicol; Clusterin overexpression as a potential neuroprotective response to the pathological effects of high fat dieting on the brain reward system.; Authors - Carmen Rodríguez-Rivera

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

George (Verified Customer) (12-03-2018)

IHC performed on paraffin-embedded human kidney tissue

• Applications: Immunohistochemistry,
• Primary Antibody Dilution: 1:200
• Cell Tissue Type: human

Clusterin Antibody Immunohistochemistry, validation (1:200 dilution) in human (Cat no:12289-1-AP)

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