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Anticorps Monoclonal anti-Clusterin

Clusterin Monoclonal Antibody for IHC, WB,ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC,ELISA

Conjugaison

Non conjugué

CloneNo.

2F12G10

N° de cat : 66109-1-Ig

Synonymes

AAG4, Apo J, APOJ, ApoJalpha, ApoJbeta, Apolipoprotein J, CLI, CLU, clusterin, Complement cytolysis inhibitor, Ku70 binding protein 1, KUB1, NA1/NA2, SGP 2, SGP2, SP 40, TRPM 2, TRPM2

Galerie de données de validation

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  • Western Blot (WB) analysis of human plasma using Clusterin Monoclonal antibody (66109-1-Ig)

    WB analysis of human plasma using 66109-1-Ig

    human plasma were subjected to SDS PAGE followed by western blot with 66109-1-Ig (Clusterin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

  • Immunohistochemistry (IHC) staining of human liver tissue using Clusterin Monoclonal antibody (66109-1-Ig)

    IHC staining of human liver using 66109-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver slide using 66109-1-Ig (Clusterin antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human liver tissue using Clusterin Monoclonal antibody (66109-1-Ig)

    IHC staining of human liver using 66109-1-Ig

    Immunohistochemical analysis of paraffin-embedded human liver slide using 66109-1-Ig (Clusterin antibody at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Clusterin Monoclonal antibody (66109-1-Ig)

    IHC staining of human breast cancer using 66109-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 66109-1-Ig (Clusterin antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human breast cancer tissue using Clusterin Monoclonal antibody (66109-1-Ig)

    IHC staining of human breast cancer using 66109-1-Ig

    Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 66109-1-Ig (Clusterin antibody at dilution of 1:50 (under 40x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Clusterin Monoclonal antibody (66109-1-Ig)

    IHC staining of human prostate cancer using 66109-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 66109-1-Ig (Clusterin antibody at dilution of 1:50 (under 10x lens).

  • Immunohistochemistry (IHC) staining of human prostate cancer tissue using Clusterin Monoclonal antibody (66109-1-Ig)

    IHC staining of human prostate cancer using 66109-1-Ig

    Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 66109-1-Ig (Clusterin antibody at dilution of 1:50 (under 40x lens).

Applications testées

Résultats positifs en WBplasma humain,
Résultats positifs en IHCtissu hépatique humain, tissu de cancer de la prostate humain, tissu de cancer du sein humain
il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:5000-1:50000
Immunohistochimie (IHC)IHC : 1:20-1:200
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

66109-1-Ig cible Clusterin dans les applications de WB, IHC,ELISA et montre une réactivité avec des échantillons Humain

Réactivité Humain
Hôte / Isotype Mouse / IgG1
Clonalité Monoclonal
Type Anticorps
Immunogène Clusterin Protéine recombinante Ag19236
Nom complet clusterin
Masse moléculaire calculée 449 aa, 53 kDa
Poids moléculaire observé 36 kDa
Numéro d’acquisition GenBankBC010514
Symbole du gène CLU
Identification du gène (NCBI) 1191
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par protéine G
Tampon de stockage PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Clusterin (Apolipoprotein J) is a 75-80 kDa heterodimeric glycoprotein produced by a wide array of tissues and found in most biologic fluids (PMID: 7648419; 11906815). It is encoded by one single gene and cleaved posttranslationally into α (34-36 kDa) and β (36-39 kDa) subunits prior to secretion from the cell. Many functions have been described for clusterin, including regulating apoptosis, transporting lipids, controlling cell interactions, regulating complement (PMID: 10694874).

Protocole

Product Specific Protocols
WB protocol for Clusterin antibody 66109-1-IgDownload protocol
IHC protocol for Clusterin antibody 66109-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols