Anticorps Monoclonal anti-Clusterin

Clusterin Monoclonal Antibody for WB, IHC, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain

Applications

WB, IHC, ELISA

Conjugaison

Non conjugué

CloneNo.

2F12G10

Avis (1)

N° de cat : 66109-1-Ig

Synonymes

Apo-J, APOJ, Apo J, Aging-associated gene 4 protein, AAG4

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Galerie de données de validation

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  WB analysis of human plasma using 66109-1-Ig

  human plasma were subjected to SDS PAGE followed by western blot with 66109-1-Ig (Clusterin antibody) at dilution of 1:20000 incubated at room temperature for 1.5 hours.

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  IHC staining of human liver using 66109-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver slide using 66109-1-Ig (Clusterin antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human liver using 66109-1-Ig

  Immunohistochemical analysis of paraffin-embedded human liver slide using 66109-1-Ig (Clusterin antibody at dilution of 1:50 (under 40x lens).

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  IHC staining of human breast cancer using 66109-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 66109-1-Ig (Clusterin antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human breast cancer using 66109-1-Ig

  Immunohistochemical analysis of paraffin-embedded human breast cancer slide using 66109-1-Ig (Clusterin antibody at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human prostate cancer using 66109-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 66109-1-Ig (Clusterin antibody at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate cancer using 66109-1-Ig

  Immunohistochemical analysis of paraffin-embedded human prostate cancer slide using 66109-1-Ig (Clusterin antibody at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: plasma humain,
• Résultats positifs en IHC: tissu hépatique humain, tissu de cancer de la prostate humain, tissu de cancer du sein humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:5000-1:50000
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

66109-1-Ig cible Clusterin dans les applications de WB, IHC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: Clusterin Protéine recombinante Ag19236
• Nom complet: clusterin
• Masse moléculaire calculée: 449 aa, 53 kDa
• Poids moléculaire observé: 36 kDa
• Numéro d’acquisition GenBank: BC010514
• Symbole du gène: CLU
• Identification du gène (NCBI): 1191
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Clusterin (Apolipoprotein J) is a 75-80 kDa heterodimeric glycoprotein produced by a wide array of tissues and found in most biologic fluids (PMID: 7648419; 11906815). It is encoded by one single gene and cleaved posttranslationally into α (34-36 kDa) and β (36-39 kDa) subunits prior to secretion from the cell. Many functions have been described for clusterin, including regulating apoptosis, transporting lipids, controlling cell interactions, regulating complement (PMID: 10694874).

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Christine (Verified Customer) (08-05-2025)

Detects 2 bands in total cell lysates of HEK293T (between 25 and 35 kDa) but nothing in the supernatant (I was expecting its secretion), so I am not sure whether it works or not

• Applications: Western Blot
• Primary Antibody Dilution: 1:2,000

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