Anticorps Polyclonal de lapin anti-Mast Cell Chymase

• Phare
• Validé par KD/KO

Mast Cell Chymase Polyclonal Antibody for IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

11

N° de cat : 18189-1-AP

Synonymes

Alpha chymase, chymase, chymase 1, mast cell, CMA1, CYH, CYM, Mast Cell Chymase, Mast cell protease I, Mcpt 5, Mcpt5, MCT1

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Galerie de données de validation

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  WB analysis of mouse heart using 18189-1-AP

  mouse heart tissue were subjected to SDS PAGE followed by western blot with 18189-1-AP (Mast Cell Chymase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of MCF-7 using 18189-1-AP

  MCF7 cells were subjected to SDS PAGE followed by western blot with 18189-1-AP (Mast Cell Chymase antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human heart using 18189-1-AP

  human heart tissue were subjected to SDS PAGE followed by western blot with 18189-1-AP (Mast Cell Chymase antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of human placenta using 18189-1-AP

  human placenta tissue were subjected to SDS PAGE followed by western blot with 18189-1-AP (Mast Cell Chymase antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

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  IP experiment of mouse heart using 18189-1-AP

  IP Result of anti-Mast Cell Chymase (IP:18189-1-AP, 4ug; Detection:18189-1-AP 1:300) with mouse heart tissue lysate 2400ug.

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  IHC staining of human tonsillitis using 18189-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 18189-1-AP (Mast Cell Chymase antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human tonsillitis using 18189-1-AP

  Immunohistochemical analysis of paraffin-embedded human tonsillitis tissue slide using 18189-1-AP (Mast Cell Chymase antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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Applications testées

• Résultats positifs en WB: tissu cardiaque de souris, cellules MCF-7, tissu cardiaque humain, tissu placentaire humain
• Résultats positifs en IP: tissu cardiaque de souris
• Résultats positifs en IHC: tissu d'amygdalite humain, ; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:1000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 9 publications below
• IF: See 2 publications below

Informations sur le produit

18189-1-AP cible Mast Cell Chymase dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: Mast Cell Chymase Protéine recombinante Ag12909
• Nom complet: chymase 1, mast cell
• Masse moléculaire calculée: 247 aa, 27 kDa
• Poids moléculaire observé: 27-30 kDa
• Numéro d’acquisition GenBank: BC069370
• Symbole du gène: CMA1
• Identification du gène (NCBI): 1215
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Mast Cell Chymase, also named as CYH, CYM and CMA1, belongs to the peptidase S1 family and Granzyme subfamily. It is the major secreted protease of mast cells with suspected roles in vasoactive peptide generation, extracellular matrix degradation, and regulation of gland secretion. CMA1 maybe a target for cardiovascular disease therapies.

Publications

Species	Application	Title
mouse	WB	Proc Natl Acad Sci U S A; Perinatal androgens organize sex differences in mast cells and attenuate anaphylaxis severity into adulthood.; Authors - Emily Mackey
human	WB	Arterioscler Thromb Vasc Biol; Stabilization of Perivascular Mast Cells by Endothelial CNP (C-Type Natriuretic Peptide).; Authors - Wen Chen
human		Int J Mol Sci; Stress and Nasal Allergy: Corticotropin-Releasing Hormone Stimulates Mast Cell Degranulation and Proliferation in Human Nasal Mucosa.; Authors - Mika Yamanaka-Takaichi
human	WB	Cell Cycle; Overexpression of monocarboxylate anion transporter 1 and 4 in T24-induced cancer-associated fibroblasts regulates the progression of bladder cancer cells in a 3D microfluidic device.; Authors - Haoqing Shi
mouse	WB	Sci Rep; Chymase-producing cells of the innate immune system are required for decidual vascular remodeling and fetal growth.; Authors - Nicole Meyer
mouse	WB	Immunology; The circadian clock is functional in eosinophils and mast cells.; Authors - Baumann Anja A