Anticorps Polyclonal de lapin anti-CMTM8

CMTM8 Polyclonal Antibody for IHC, IP, WB,ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, ELISA

Conjugaison

Non conjugué

Publications

5

N° de cat : 15039-1-AP

Synonymes

CKLFSF8, CKLFSF8 V2, CMTM8

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Galerie de données de validation

• 

  WB analysis of mouse liver using 15039-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15039-1-AP (CMTM8 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse liver using 15039-1-AP

  mouse liver tissue were subjected to SDS PAGE followed by western blot with 15039-1-AP (CMTM8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  WB analysis of mouse pancreas using 15039-1-AP

  mouse pancreas tissue were subjected to SDS PAGE followed by western blot with 15039-1-AP (CMTM8 antibody) at dilution of 1:300 incubated at room temperature for 1.5 hours.

• 

  IP experiment of mouse liver using 15039-1-AP

  IP Result of anti-CMTM8 (IP:15039-1-AP, 4ug; Detection:15039-1-AP 1:300) with mouse liver tissue lysate 6800ug.

• 

  IHC staining of human liver using 15039-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15039-1-AP (CMTM8 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver using 15039-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 15039-1-AP (CMTM8 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas cancer using 15039-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 15039-1-AP (CMTM8 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas cancer using 15039-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas cancer using 15039-1-AP (CMTM8 antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human pancreas using 15039-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 15039-1-AP (CMTM8 antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human pancreas using 15039-1-AP

  Immunohistochemical analysis of paraffin-embedded human pancreas using 15039-1-AP (CMTM8 antibody) at dilution of 1:50 (under 40x lens).

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Applications testées

• Résultats positifs en WB: tissu hépatique de souris, tissu pancréatique de souris
• Résultats positifs en IP: tissu hépatique de souris
• Résultats positifs en IHC: tissu hépatique humain, tissu de cancer du pancréas humain, tissu pancréatique humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:20-1:200

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IHC: See 3 publications below

Informations sur le produit

15039-1-AP cible CMTM8 dans les applications de WB, IP, IHC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CMTM8 Protéine recombinante Ag6776
• Nom complet: CKLF-like MARVEL transmembrane domain containing 8
• Masse moléculaire calculée: 20 kDa
• Poids moléculaire observé: 25-28 kDa
• Numéro d’acquisition GenBank: BC041390
• Symbole du gène: CMTM8
• Identification du gène (NCBI): 152189
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CMTM8, also known as CKLFSF8, is a member of the chemokine-like factor superfamily (CKLFSF). Proteins of this family share similarity with both the chemokine and the transmembrane 4 superfamilies. CMTM8 is an attenuator of EGF-induced signaling (PMID: 16263120). Overexpression of CMTM8 accelerates ligand-induced clearance of EGFR from the cell surface and induces apoptosis via caspase-dependent and -independent pathways (PMID: 17149703). An alternative splice form of CMTM8, CMTM8-v2, arises from a deletion of exon 2. CMTM8-v2 maintains the ability to induce apoptosis (PMID: 17681841).

Publications

Species	Application	Title
human	IHC	DNA Cell Biol; Downregulated CMTM8 Correlates with Poor Prognosis in Gastric Cancer Patients.; Authors - Meng Yan
human	IHC	BMC Gastroenterol; Identification and validation of EMT-immune-related prognostic biomarkers CDKN2A, CMTM8 and ILK in colon cancer.; Authors - Ning Kang
human	WB	Oncol Rep; CMTM8 inhibits the carcinogenesis and progression of bladder cancer.; Authors - Denghui Gao
human	WB	Bioengineered; MicroRNA-582-5p promotes triple-negative breast cancer invasion and metastasis by antagonizing CMTM8; Authors - Xue Zeng
	IHC	Cancers (Basel); The MAL Family of Proteins: Normal Function, Expression in Cancer, and Potential Use as Cancer Biomarkers; Authors - Leticia Labat-de-Hoz

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