Anticorps Polyclonal de lapin anti-CNN2

• Phare
• Validé par KD/KO

CNN2 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(6)

N° de cat : 21073-1-AP

Synonymes

calponin 2, Calponin H2, smooth muscle, CNN2, Neutral calponin

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Galerie de données de validation

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  WB analysis of HeLa using 21073-1-AP

  WB result of CNN2 antibody (21073-1-AP; 1:100000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNN2 transfected HeLa cells.

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  WB analysis using 21073-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 21073-1-AP (CNN2 antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IHC staining of human heart using 21073-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 21073-1-AP (CNN2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human heart using 21073-1-AP

  Immunohistochemical analysis of paraffin-embedded human heart using 21073-1-AP (CNN2 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human hysteromyoma using 21073-1-AP

  Immunohistochemical analysis of paraffin-embedded human hysteromyoma using 21073-1-AP (CNN2 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human hysteromyoma using 21073-1-AP

  Immunohistochemical analysis of paraffin-embedded human hysteromyoma using 21073-1-AP (CNN2 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HepG2 using 21073-1-AP

  Immunofluorescent analysis of (-20°C Methanol) fixed HepG2 cells using CNN2 antibody (21073-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 21073-1-AP

  Immunofluorescent analysis of HepG2 cells, using CNN2 antibody 21073-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red).

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Applications testées

• Résultats positifs en WB: cellules HeLa, cellules HepG2
• Résultats positifs en IHC: tissu cardiaque humain, tissu d'hystéromyome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunohistochimie (IHC): IHC : 1:20-1:200
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 2 publications below
• WB: See 4 publications below
• IHC: See 2 publications below
• IF: See 2 publications below

Informations sur le produit

21073-1-AP cible CNN2 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CNN2 Protéine recombinante Ag15225
• Nom complet: calponin 2
• Masse moléculaire calculée: 309 aa, 34 kDa
• Poids moléculaire observé: 34-36 kDa
• Numéro d’acquisition GenBank: BC141818
• Symbole du gène: CNN2
• Identification du gène (NCBI): 1265
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Calponin is a family of actin filament-associated proteins which regulate smooth muscle cell contraction. Three isoforms of calponin exist: calponin h1 (CNN1) , calponin h2 (CNN2) and calponin 3 (CNN3). Calponin 1 and calponin 2 are predominately expressed in smooth muscle cells and cardiac muscle cells, respectively. Calponin 3 is highly expressed in many tissues including articular cartilage and brain.

Publications

Species	Application	Title
mouse	IF	Circ Res; Mitochondrial Protein Poldip2 Controls VSMC Differentiated Phenotype by O-Linked GlcNAc Transferase-Dependent Inhibition of a Ubiquitin Proteasome System.; Authors - Felipe Paredes
human	IHC, WB	iScience; Inhibitor of Differentiation 4 (ID4) represses mammary myoepithelial differentiation via inhibition of HEB.; Authors - Holly Holliday
rat	WB	Front Pharmacol; Activation of the Cholinergic Anti-inflammatory Pathway Attenuated Angiotension II-Dependent Hypertension and Renal Injury.; Authors - Shu-Jie Wu
human	WB	Tumour Biol; Knockdown of calponin 2 suppressed cell growth in gastric cancer cells.; Authors - Jianwei Hu ; KD Validated
mouse	IF	Elife; Differential regulation of hair cell actin cytoskeleton mediated by SRF and MRTFB; Authors - Ling-Yun Zhou
mouse,rat	WB,IHC	Mol Metab; Calponin 2 harnesses metabolic reprogramming to determine kidney fibrosis; Authors - Yuan Gui ; KD Validated

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