Anticorps Polyclonal de lapin anti-CNN3

• Phare
• Validé par KD/KO

CNN3 Polyclonal Antibody for IF, IHC, IP, WB, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris

Applications

WB, IP, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications

3

N° de cat : 11509-1-AP

Synonymes

Calponin 3, calponin 3, acidic, Calponin, acidic isoform, CNN3

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Galerie de données de validation

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  WB analysis of HEK-293 using 11509-1-AP

  WB result of CNN3 antibody (11509-1-AP; 1:1000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNN3 transfected HEK-293 cells.

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  WB analysis using 11509-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 11509-1-AP (CNN3 antibody) at dilution of 1:3000 incubated at room temperature for 1.5 hours.

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  WB analysis of HAP1 cells using 11509-1-AP

  HAP1 (WT and CNN3 KO) lysates prepared with RIPA buffer, 20 μg of protein loaded. 11509-1-AP incubated at 1:100 at 4°C overnight in 5% milk in TBST. Ponceau stained transfers shown for each blot. These data were performed and provided by YCharOS in partnership with Proteintech. YCharOS is an open science company with a mission to characterise all commercial antibodies to improve scientific reproducibility and transparency.

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  WB analysis of human kidney using 11509-1-AP

  human kidney tissue were subjected to SDS PAGE followed by western blot with 11509-1-AP (CNN3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  WB analysis of human skeletal muscle using 11509-1-AP

  human skeletal muscle tissue were subjected to SDS PAGE followed by western blot with 11509-1-AP (CNN3 antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

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  IP experiment of U-87MG cells using 11509-1-AP

  U-87MG lysates prepared and IP of APP performed using 2.0 μg of 11509-1-AP coupled to protein A- Dynabeads beads. The Ponceau stained transfers of each blot are shown. SM=4% starting material; UB=4% unbound fraction; IP=immunoprecipitated; HC = antibody heavy chain. These data were performed and provided by YCharOS in partnership with Proteintech. YCharOS is an open science company with a mission to characterise all commercial antibodies to improve scientific reproducibility and transparency.

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  IP experiment of HEK-293 using 11509-1-AP

  IP result of anti-CNN3(IP:11509-1-AP, 4ug; Detection:11509-1-AP 1:15000) with HEK-293 cells lysate 960 ug.

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  IHC staining of human prostate cancer using 11509-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11509-1-AP (CNN3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human prostate cancer using 11509-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11509-1-AP (CNN3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 11509-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11509-1-AP (CNN3 antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach cancer using 11509-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11509-1-AP (CNN3 antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human skin using 11509-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 11509-1-AP (CNN3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human skin using 11509-1-AP

  Immunohistochemical analysis of paraffin-embedded human skin using 11509-1-AP (CNN3 antibody) at dilution of 1:50 (under 40x lens).

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  IHC staining of human hysteromyoma using 11509-1-AP

  Immunohistochemical analysis of paraffin-embedded human hysteromyoma using 11509-1-AP (CNN3 antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human hysteromyoma using 11509-1-AP

  Immunohistochemical analysis of paraffin-embedded human hysteromyoma using 11509-1-AP (CNN3 antibody) at dilution of 1:50 (under 40x lens).

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  IF Staining of HeLa using 11509-1-AP

  Immunofluorescent analysis of Hela cells, using CNN3 antibody 11509-1-AP at 1:25 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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Applications testées

• Résultats positifs en WB: cellules HCT 116, cellules HEK-293, cellules HepG2, cellules NIH/3T3, tissu de muscle squelettique humain, tissu rénal humain
• Résultats positifs en IP: cellules HEK-293, U-87MG cells
• Résultats positifs en IHC: tissu de cancer de la prostate humain, tissu cutané humain, tissu de cancer de l'estomac humain, tissu d'hystéromyome humain; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF: cellules HeLa

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:6000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF): IF : 1:10-1:100

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

11509-1-AP cible CNN3 dans les applications de WB, IP, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: CNN3 Protéine recombinante Ag2068
• Nom complet: calponin 3, acidic
• Masse moléculaire calculée: 329 aa, 36 kDa
• Poids moléculaire observé: 36 kDa
• Numéro d’acquisition GenBank: BC025372
• Symbole du gène: CNN3
• Identification du gène (NCBI): 1266
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
mouse	IF	Cell Rep; Spatiotemporal dynamics of inner ear sensory and non-sensory cells revealed by single-cell transcriptomics.; Authors - Taha A Jan
human	WB	J Proteome Res; Comparative Proteomics Reveals Prolonged Corneal Preservation Impaired Ocular Surface Immunity Accompanied by Fibrosis in Human Stroma; Authors - Pei-Shan Wu
human	WB	Gastroenterol Res Pract; PMID: 30911294; Authors - Keun-Seok Hong ; KD Validated