Anticorps Monoclonal anti-CNPase

• Phare
• Validé par KD/KO

CNPase Monoclonal Antibody for FC, IF, WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris

Applications

WB, IF, FC, ELISA

Conjugaison

Non conjugué

Publications

2

CloneNo.

1B9G9

N° de cat : 66729-1-Ig

Synonymes

CNP, CNP1, CNPase

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Galerie de données de validation

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  WB analysis using 66729-1-Ig

  Various lysates were subjected to SDS PAGE followed by western blot with 66729-1-Ig (CNPase antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of HepG2 using 66729-1-Ig

  WB result of CNPase antibody (66729-1-Ig; 1:10000; incubated at room temperature for 1.5 hours) with sh-Control and sh-CNPase transfected HepG2 cells.

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  WB analysis of U-251 using 66729-1-Ig

  U-251 cells were subjected to SDS PAGE followed by western blot with 66729-1-Ig (CNPase antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  WB analysis of human brain using 66729-1-Ig

  human brain tissue were subjected to SDS PAGE followed by western blot with 66729-1-Ig (CNPase antibody) at dilution of 1:5000 incubated at room temperature for 1.5 hours.

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  IF Staining of mouse brain using 66729-1-Ig

  Immunofluorescent analysis of (4% PFA) fixed mouse brain tissue using 66729-1-Ig (CNPase antibody), at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L).

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  FC experiment of HepG2 using 66729-1-Ig

  1X10^6 HepG2 cells were intracellularly stained with 0.5 ug Anti-Human CNPase (66729-1-Ig, Clone:1B9G9) and CoraLite®488-Conjugated AffiniPure Goat Anti-Mouse IgG(H+L) at dilution 1:1000 (red), or 0.5 ug Control Antibody. Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: tissu de cervelet de porc, cellules HepG2, cellules U-251, tissu cérébral de rat, tissu cérébral humain, tissu de cervelet de rat
• Résultats positifs en IF: tissu cérébral de souris,
• Résultats positifs en cytométrie: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:2000-1:10000
• Immunofluorescence (IF): IF : 1:50-1:500
• Flow Cytometry (FC): FC : 0.50 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 2 publications below
• IF: See 1 publications below

Informations sur le produit

66729-1-Ig cible CNPase dans les applications de WB, IF, FC, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

• Réactivité: Humain, porc, rat, souris
• Réactivité citée: rat, souris
• Hôte / Isotype: Mouse / IgG1
• Clonalité: Monoclonal
• Type: Anticorps
• Immunogène: CNPase Protéine recombinante Ag4227
• Nom complet: 2',3'-cyclic nucleotide 3' phosphodiesterase
• Masse moléculaire calculée: 421 aa, 48 kDa
• Poids moléculaire observé: 45-48 kDa
• Numéro d’acquisition GenBank: BC028040
• Symbole du gène: CNP
• Identification du gène (NCBI): 1267
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par protéine G
• Tampon de stockage: PBS avec azoture de sodium à 0,02 % et glycérol à 50 % pH 7,3
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

CNP(2',3'-cyclic-nucleotide 3'-phosphodiesterase) belongs to the cyclic nucleotide phosphodiesterase family. It plays an essential role in axonal survival but not in myelin assembly, coupling oligodendroglial functions in axonal support and myelination. This protein has 2 isoforms produced by alternative splicing with molecular mass of 45 and 48 kDa.

Publications

Species	Application	Title
mouse	IF,WB	CNS Neurosci Ther; EAAT3 impedes oligodendrocyte remyelination in chronic cerebral hypoperfusion-induced white matter injury; Authors - Yingmei Zhang
rat	WB	J Ethnopharmacol; Shenzhiling oral liquid protects the myelin sheath against Alzheimer's disease through the PI3K/Akt-mTOR pathway.; Authors - Mingcui Zheng