Anticorps Monoclonal anti-Collagen Type I

Collagen Type I Monoclonal Antibody for WB, ELISA

Hôte / Isotype

Mouse / IgG1

Réactivité testée

Humain, porc, rat, souris et plus (2)

Applications

WB, IHC, IF, ELISA

Conjugaison

Non conjugué

Publications(125)

CloneNo.

3D5E8

N° de cat : 66761-1-Ig

Synonymes

COL1, COL1A2, 3D5E8, Alpha-2 type I collagen, col I



Applications testées

Résultats positifs en WBtissu cutané de rat, tissu cutané de porc, tissu de queue de souris

Dilution recommandée

ApplicationDilution
Western Blot (WB)WB : 1:2000-1:10000
It is recommended that this reagent should be titrated in each testing system to obtain optimal results.
Sample-dependent, check data in validation data gallery

Informations sur le produit

66761-1-Ig cible Collagen Type I dans les applications de WB, IHC, IF, ELISA et montre une réactivité avec des échantillons Humain, porc, rat, souris

Réactivité Humain, porc, rat, souris
Réactivité citéerat, bovin, Humain, Lapin, souris
Hôte / Isotype Mouse / IgG1
Clonalité Monoclonal
Type Anticorps
Immunogène Collagen Type I Protéine recombinante Ag6281
Nom complet collagen, type I, alpha 2
Masse moléculaire calculée 1366 aa, 130 kDa
Poids moléculaire observé 140 kDa, 70-80 kDa
Numéro d’acquisition GenBankBC054498
Symbole du gène COL1A2
Identification du gène (NCBI) 1278
Conjugaison Non conjugué
Forme Liquide
Méthode de purification Purification par protéine G
Tampon de stockage PBS with 0.02% sodium azide and 50% glycerol
Conditions de stockageStocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Type I collagen, the major structural component of connective tissues such as skin, tendon and bone, is the most abundant and widely expressed collagen in humans (PMID: 7620364; 8645190; 9016532). Type I collagen is a heterotrimer comprising one alpha 2(I) and two alpha 1(I) chains which are encoded by the unlinked loci COL1A2 and COL1A1 respectively. Type I collagen has a molecular mass of about 250-300 kDa, while the alpha 2(I) chain has a molecular weight of about 100-140 kDa. Mutations in COL1A2 gene are associated with osteogenesis imperfecta, Ehlers-Danlos syndrome, idiopathic osteoporosis, and atypical Marfan syndrome. When MMPs degrade type I collagen, the major degradation products are the typical ¾ and ¼ collagen fragments with molecular weights of ~ 50-70 kDa, and ~ 25 (PMID: 8910479).

Protocole

Product Specific Protocols
WB protocol for Collagen Type I antibody 66761-1-IgDownload protocol
Standard Protocols
Click here to view our Standard Protocols

Publications

SpeciesApplicationTitle
mouseWB

Acta Pharm Sin B

Celastrol induces ferroptosis in activated HSCs to ameliorate hepatic fibrosis via targeting peroxiredoxins and HO-1.

Authors - Piao Luo
ratWB

Adv Healthc Mater

Metformin Carbon Dots for Promoting Periodontal Bone Regeneration via Activation of ERK/AMPK Pathway.

Authors - Chunxia Ren
mouseWB

Clin Transl Med

Histone methyltransferase KMT2A promotes pulmonary fibrogenesis via targeting pro-fibrotic factor PU.1 in fibroblasts

Authors - Wenting Lyu
mouseIF

Cell Mol Life Sci

Dysregulated cholesterol uptake and efflux of bone marrow-derived α-SMA+ macrophages contribute to atherosclerotic plaque formation

Authors - Menglu Li
ratWB

Phytomedicine

β-elemene alleviates hyperglycemia-induced cardiac inflammation and remodeling by inhibiting the JAK/STAT3-NF-κB pathway

Authors - Jiong Wang
ratWB,IHC

Oxid Med Cell Longev

Eugenol-Preconditioned Mesenchymal Stem Cell-Derived Extracellular Vesicles Promote Antioxidant Capacity of Tendon Stem Cells In Vitro and In Vivo.

Authors - Xiangze Li

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.


FH

Su (Verified Customer) (03-18-2022)

Works good and consistent.

  • Applications: Immunohistochemistry
  • Primary Antibody Dilution: 1:250
  • Cell Tissue Type: Cancer tissue
Collagen Type I Antibody Immunohistochemistry validation (1:250 dilution) in Cancer tissue (Cat no:66761-1-Ig)
{{ptg:RelatedPrimaryAntibodies}}