Anticorps Polyclonal de lapin anti-COMT

COMT Polyclonal Antibody for WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, rat, souris et plus (1)

Applications

WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA

Conjugaison

Non conjugué

Publications(14)

N° de cat : 14754-1-AP

Synonymes

catechol O methyltransferase, Catechol O-methyltransferase, EC:2.1.1.6

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Galerie de données de validation

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  WB analysis using 14754-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 14754-1-AP (COMT antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  IP experiment of K-562 using 14754-1-AP

  IP result of anti-COMT (IP:14754-1-AP, 4ug; Detection:14754-1-AP 1:500) with K-562 cells lysate 1200ug.

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  IHC staining of human liver using 14754-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver using 14754-1-AP (COMT antibody) at dilution of 1:100 (under 40x lens).

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  IHC staining of human placenta using 14754-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14754-1-AP (COMT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human placenta using 14754-1-AP

  Immunohistochemical analysis of paraffin-embedded human placenta tissue slide using 14754-1-AP (COMT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 14754-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 14754-1-AP (COMT antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of mouse lung using 14754-1-AP

  Immunohistochemical analysis of paraffin-embedded mouse lung tissue slide using 14754-1-AP (COMT antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of mouse cerebellum using 14754-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using COMT antibody (14754-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of mouse cerebellum using 14754-1-AP

  Immunofluorescent analysis of (4% PFA) fixed mouse cerebellum tissue using COMT antibody (14754-1-AP) at dilution of 1:200 and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L).

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  IF Staining of HepG2 using 14754-1-AP

  Immunofluorescent analysis of HepG2 cells, using COMT antibody 14754-1-AP at 1:50 dilution and Rhodamine-labeled goat anti-rabbit IgG (red). Blue pseudocolor = DAPI (fluorescent DNA dye).

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  FC experiment of PC-3 using 14754-1-AP

  1x10^6 PC-3 cells were intracellularly stained with 0.8 ug COMT Polyclonal antibody (14754-1-AP) and CoraLite®488-Conjugated AffiniPure Goat Anti-Rabbit IgG(H+L) (SA00013-2)(red), or 0.8 ug rabbit IgG isotype control (blue). Cells were fixed with 4% PFA and permeabilized with Flow Cytometry Perm Buffer (PF00011-C).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules K-562, foie de rat, foie de souris, placenta humain
• Résultats positifs en IP: cellules K-562
• Résultats positifs en IHC: tissu hépatique humain, tissu placentaire humain, tissu pulmonaire de souris; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF-P: tissu de cervelet de souris,
• Résultats positifs en IF/ICC: cellules HepG2,
• Résultats positifs en FC (Intra): cellules PC-3,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunoprécipitation (IP): IP : 0.5-4.0 ug for 1.0-3.0 mg of total protein lysate
• Immunohistochimie (IHC): IHC : 1:100-1:800
• Immunofluorescence (IF)-P: IF-P : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:50-1:500
• Flow Cytometry (FC) (INTRA): FC (INTRA) : 0.80 ug per 10^6 cells in a 100 µl suspension

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• WB: See 12 publications below
• IHC: See 2 publications below
• IF: See 3 publications below

Informations sur le produit

14754-1-AP cible COMT dans les applications de WB, IHC, IF/ICC, IF-P, FC (Intra), IP, ELISA et montre une réactivité avec des échantillons Humain, rat, souris

• Réactivité: Humain, rat, souris
• Réactivité citée: rat, Humain, singe, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: COMT Protéine recombinante Ag6502
• Nom complet: catechol-O-methyltransferase
• Masse moléculaire calculée: 30 kDa
• Poids moléculaire observé: 28-30 kDa
• Numéro d’acquisition GenBank: BC000419
• Symbole du gène: COMT
• Identification du gène (NCBI): 1312
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Informations générales

Catechol O-methyltransferase (COMT) is an important enzyme that catalyzes O-methylation and inactivation of metabolically active catechol-containing bioactive molecules and drugs. The mammalian COMT enzyme exists in soluble form (24-26 kDa) and membrane-bound form (28-30 kDa), which are encoded by a single gene utilizing different promoters.

Publications

Species	Application	Title
human	WB	Adv Sci (Weinh); Meisoindigo Acts as a Molecular Glue to Target PKMYT1 for Degradation in Chronic Myeloid Leukemia Therapy; Authors - Zhao-Xin Zhang
mouse	WB	Aging (Albany NY); A five-m6A regulatory gene signature is a prognostic biomarker in lung adenocarcinoma patients.; Authors - Xiao Wu
mouse	WB	Aging (Albany NY); Maternal high sugar and fat diet benefits offspring brain function via targeting on the gut-brain axis.; Authors - Dongdong Wang
mouse	WB	Transl Psychiatry; A diet high in sugar and fat influences neurotransmitter metabolism and then affects brain function by altering the gut microbiota.; Authors - Yinrui Guo
mouse	WB	FASEB J; Entacapone alleviates acute kidney injury by inhibiting ferroptosis.; Authors - Jiahong Yang
human	WB,IHC	Endocrinology; Norepinephrine, active norepinephrine transporter, and norepinephrine-metabolism are involved in the generation of reactive oxygen species in human ovarian granulosa cells.; Authors - Saller S S

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