Anticorps Polyclonal de lapin anti-COPE

COPE Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

N° de cat : 11457-1-AP

Synonymes

Epsilon-COP, Epsilon-coat protein, Coatomer subunit epsilon

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Galerie de données de validation

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  WB analysis of HEK-293 using 11457-1-AP

  HEK-293 cells were subjected to SDS PAGE followed by western blot with 11457-1-AP (COPE antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

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  WB analysis of A431 using 11457-1-AP

  A431 cells were subjected to SDS PAGE followed by western blot with 11457-1-AP (COPE antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of MCF-7 using 11457-1-AP

  MCF-7 cells were subjected to SDS PAGE followed by western blot with 11457-1-AP (COPE antibody) at dilution of 1:2000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of A375 using 11457-1-AP

  A375 cells were subjected to SDS PAGE followed by western blot with 11457-1-AP (COPE antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

• 

  WB analysis of HeLa using 11457-1-AP

  HeLa cells were subjected to SDS PAGE followed by western blot with 11457-1-AP (COPE antibody) at dilution of 1:600 incubated at room temperature for 1.5 hours.

• 

  WB analysis of PC-3 using 11457-1-AP

  PC-3 cells were subjected to SDS PAGE followed by western blot with 11457-1-AP (COPE antibody) at dilution of 1:500 incubated at room temperature for 1.5 hours.

• 

  IHC staining of human stomach cancer using 11457-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11457-1-AP (COPE antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human stomach cancer using 11457-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach cancer tissue slide using 11457-1-AP (COPE antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of rat stomach using 11457-1-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 11457-1-AP (COPE antibody) at dilution of 1:200 (under 10x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of rat stomach using 11457-1-AP

  Immunohistochemical analysis of paraffin-embedded rat stomach tissue slide using 11457-1-AP (COPE antibody) at dilution of 1:200 (under 40x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

• 

  IHC staining of human prostate cancer using 11457-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11457-1-AP (COPE Antibody) at dilution of 1:50 (under 10x lens).

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  IHC staining of human prostate cancer using 11457-1-AP

  Immunohistochemical analysis of paraffin-embedded human prostate cancer tissue slide using 11457-1-AP (COPE Antibody) at dilution of 1:50 (under 40x lens).

• 

  IHC staining of human liver cancer using 11457-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11457-1-AP (COPE Antibody) at dilution of 1:50 (under 10x lens).

• 

  IHC staining of human liver cancer using 11457-1-AP

  Immunohistochemical analysis of paraffin-embedded human liver cancer tissue slide using 11457-1-AP (COPE Antibody) at dilution of 1:50 (under 40x lens).

• 

  IF Staining of HepG2 using 11457-1-AP

  Immunofluorescent analysis of (4% PFA) fixed HepG2 cells using COPE antibody (11457-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules HEK-293, cellules A375, cellules A431, cellules HeLa, cellules MCF-7, cellules PC-3
• Résultats positifs en IHC: tissu de cancer de l'estomac humain, tissu de cancer de la prostate humain, tissu de cancer du foie humain, tissu d'estomac de rat; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules HepG2,

Dilution recommandée

• Western Blot (WB): WB : 1:1000-1:4000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Informations sur le produit

11457-1-AP cible COPE dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain

• Réactivité: Humain
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: COPE Protéine recombinante Ag2024
• Nom complet: coatomer protein complex, subunit epsilon
• Masse moléculaire calculée: 34 kDa
• Poids moléculaire observé: 30-35 kDa
• Numéro d’acquisition GenBank: BC017285
• Symbole du gène: COPE
• Identification du gène (NCBI): 11316
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

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