Anticorps Polyclonal de lapin anti-COX16

• Phare
• Validé par KD/KO

COX16 Polyclonal Antibody for WB, IHC, IF/ICC, ELISA

Hôte / Isotype

Lapin / IgG

Réactivité testée

Humain, souris

Applications

WB, IHC, IF/ICC, ELISA

Conjugaison

Non conjugué

Publications(7)

Avis (1)

N° de cat : 19425-1-AP

Synonymes

C14orf112, COX 16, hCOX16, HSPC203, MC4DN22

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Galerie de données de validation

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  WB analysis using 19425-1-AP

  Various lysates were subjected to SDS PAGE followed by western blot with 19425-1-AP (COX16 antibody) at dilution of 1:1000 incubated at room temperature for 1.5 hours.

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  WB analysis of HeLa using 19425-1-AP

  WB result of COX16 antibody (19425-1-AP; 1:600; incubated at room temperature for 1.5 hours) with sh-Control and sh-COX16 transfected HeLa cells.

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  IHC staining of human ovary cancer using 19425-1-AP

  Immunohistochemical analysis of paraffin-embedded human ovary cancer tissue slide using 19425-1-AP (COX16 antibody) at dilution of 1:100 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IHC staining of human stomach using 19425-1-AP

  Immunohistochemical analysis of paraffin-embedded human stomach tissue slide using 19425-1-AP (COX16 antibody) at dilution of 1:200 (under 20x lens). Heat mediated antigen retrieval with Tris-EDTA buffer (pH 9.0).

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  IF Staining of U2OS using 19425-1-AP

  Immunofluorescent analysis of (4% PFA) fixed U2OS cells using COX16 antibody (19425-1-AP) at dilution of 1:400 and CoraLite®488-Conjugated Goat Anti-Rabbit IgG(H+L) (SA00013-2), CL594-Phalloidin (red).

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Applications testées

• Résultats positifs en WB: cellules A549, cellules HeLa, cellules SH-SY5Y
• Résultats positifs en IHC: tissu d'estomac humain, human ovary cancer tissue; il est suggéré de démasquer l'antigène avec un tampon de TE buffer pH 9.0; (*) À défaut, 'le démasquage de l'antigène peut être 'effectué avec un tampon citrate pH 6,0.
• Résultats positifs en IF/ICC: cellules U2OS,

Dilution recommandée

• Western Blot (WB): WB : 1:500-1:2000
• Immunohistochimie (IHC): IHC : 1:50-1:500
• Immunofluorescence (IF)/ICC: IF/ICC : 1:200-1:800

It is recommended that this reagent should be titrated in each testing system to obtain optimal results.

Sample-dependent, check data in validation data gallery

Applications publiées

• KD/KO: See 1 publications below
• WB: See 7 publications below

Informations sur le produit

19425-1-AP cible COX16 dans les applications de WB, IHC, IF/ICC, ELISA et montre une réactivité avec des échantillons Humain, souris

• Réactivité: Humain, souris
• Réactivité citée: Humain, souris
• Hôte / Isotype: Lapin / IgG
• Clonalité: Polyclonal
• Type: Anticorps
• Immunogène: COX16 Protéine recombinante Ag13756
• Nom complet: COX16 cytochrome c oxidase assembly homolog (S. cerevisiae)
• Masse moléculaire calculée: 106 aa, 12 kDa
• Poids moléculaire observé: 12 kDa
• Numéro d’acquisition GenBank: BC001702
• Symbole du gène: COX16
• Identification du gène (NCBI): 51241
• Conjugaison: Non conjugué
• Forme: Liquide
• Méthode de purification: Purification par affinité contre l'antigène
• Tampon de stockage: PBS with 0.02% sodium azide and 50% glycerol
• Conditions de stockage: Stocker à -20°C. Stable pendant un an après l'expédition. L'aliquotage n'est pas nécessaire pour le stockage à -20^oC Les 20ul contiennent 0,1% de BSA.

Publications

Species	Application	Title
human	WB	Mol Cell; Global mitochondrial protein import proteomics reveal distinct regulation by translation and translocation machinery; Authors - Jasmin Adriana Schafer
human	WB	Nat Commun; Coordination of metal center biogenesis in human cytochrome c oxidase.; Authors - Eva Nývltová
	WB	Nat Commun; The HSP90/R2TP assembly chaperone promotes cell proliferation in the intestinal epithelium.; Authors - Chloé Maurizy
human	WB	Elife; COX16 promotes COX2 metallation and assembly during respiratory complex IV biogenesis.; Authors - Abhishek Aich ; KO Validated
human	WB	J Biol Chem; An animal model for mitochondrial tyrosyl-tRNA synthetase deficiency reveals links between oxidative phosphorylation and retinal function.; Authors - Xiaofen Jin
mouse	WB	Redox Biol; Lead aggravates Alzheimer's disease pathology via mitochondrial copper accumulation regulated by COX17; Authors - Dingbang Huang

Avis

The reviews below have been submitted by verified Proteintech customers who received an incentive for providing their feedback.

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FH

Süleyman (Verified Customer) (02-07-2022)

There are some unspecific bands which were distant but stronger than COX16 itself, even though I loaded 30-40ug of protein and 1.1000 dilution of antibody, either HeLa cells have very low abundant or antibody has some problems.

• Applications: Western Blot
• Primary Antibody Dilution: 1:1000
• Cell Tissue Type: HeLa

COX16 Antibody Western Blot validation (1:1000 dilution) in HeLa (Cat no:19425-1-AP)

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